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Enzymatic deglycosylation of glycoproteins.
Kim MS, Leahy D. Kim MS, et al. Methods Enzymol. 2013;533:259-63. doi: 10.1016/B978-0-12-420067-8.00019-2. Methods Enzymol. 2013. PMID: 24182931
Generating mammalian stable cell lines by electroporation.
A Longo P, Kavran JM, Kim MS, Leahy DJ. A Longo P, et al. Among authors: leahy dj. Methods Enzymol. 2013;529:209-26. doi: 10.1016/B978-0-12-418687-3.00017-3. Methods Enzymol. 2013. PMID: 24011048 Free PMC article.
Lysis of mammalian and Sf9 cells.
Kavran JM, Leahy DJ. Kavran JM, et al. Among authors: leahy dj. Methods Enzymol. 2014;536:47-52. doi: 10.1016/B978-0-12-420070-8.00004-0. Methods Enzymol. 2014. PMID: 24423265 Free PMC article.
Single cell cloning of a stable mammalian cell line.
Longo PA, Kavran JM, Kim MS, Leahy DJ. Longo PA, et al. Among authors: leahy dj. Methods Enzymol. 2014;536:165-72. doi: 10.1016/B978-0-12-420070-8.00014-3. Methods Enzymol. 2014. PMID: 24423275
Silver staining of SDS-polyacrylamide gel.
Kavran JM, Leahy DJ. Kavran JM, et al. Among authors: leahy dj. Methods Enzymol. 2014;541:169-76. doi: 10.1016/B978-0-12-420119-4.00014-8. Methods Enzymol. 2014. PMID: 24674071
Immunoaffinity Purification of Proteins.
Kavran JM, Leahy DJ. Kavran JM, et al. Among authors: leahy dj. Methods Enzymol. 2015;559:27-36. doi: 10.1016/bs.mie.2014.11.006. Epub 2015 Mar 29. Methods Enzymol. 2015. PMID: 26096501
112 results