Single cell cloning of a stable mammalian cell line

Methods Enzymol. 2014:536:165-72. doi: 10.1016/B978-0-12-420070-8.00014-3.

Authors

Patti A Longo¹, Jennifer M Kavran¹, Min-Sung Kim¹, Daniel J Leahy²

Affiliations

¹ Johns Hopkins University School of Medicine, Baltimore, MD, USA.
² Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: dleahy@jhmi.edu.

PMID: 24423275
DOI: 10.1016/B978-0-12-420070-8.00014-3

Abstract

Isolate cell lines with improved stability or expression properties from a parental cell line.

Keywords: Clonal cell line; Dilution of cells; Protein expression; Single cell cloning; Stable mammalian cell line.

© 2014 Elsevier Inc. All rights reserved.

MeSH terms

Animals
CHO Cells
Cell Culture Techniques*
Cell Line
Cricetinae
Cricetulus
HEK293 Cells
Humans
Recombinant Proteins / biosynthesis
Sf9 Cells
Spodoptera

Substances

Recombinant Proteins