Coupling antibody to cyanogen bromide-activated sepharose

Jennifer M Kavran; Daniel J Leahy

doi:10.1016/B978-0-12-420119-4.00003-3

Coupling antibody to cyanogen bromide-activated sepharose

Methods Enzymol. 2014:541:27-34. doi: 10.1016/B978-0-12-420119-4.00003-3.

Authors

Jennifer M Kavran¹, Daniel J Leahy²

Affiliations

¹ Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
² Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA. Electronic address: dleahy@jhmi.edu.

Abstract

Antibodies will be immobilized on a cyanogen bromide-activated Sepharose for subsequent use in pull-down assays or immunoaffinity purification.

Keywords: Antibody and resin preparation; Antigen–antibody reactions; Ascitic fluid; Coupling antibody to resin; Cyanogen bromide (CNBr)-activated Sepharose; Hybridoma; Resin.

MeSH terms

Antibodies, Immobilized / chemistry*
Antibodies, Monoclonal / chemistry*
Chromatography, Affinity / instrumentation
Cyanogen Bromide / chemistry*
Resins, Synthetic / chemistry
Sepharose / chemistry*

Substances

Antibodies, Immobilized
Antibodies, Monoclonal
Resins, Synthetic
Sepharose
Cyanogen Bromide

Grants and funding

P30 CA006973/CA/NCI NIH HHS/United States