Abstract
This protocol entails a single-step, high-affinity purification of proteins using an immobilized antibody column.
Keywords:
Binding buffer; Elution buffer; Immunoaffinity purification; Immunoaffinty columns; Neutralization buffer; SDS-PAGE.
© 2015 Elsevier Inc. All rights reserved.
MeSH terms
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Antibodies / chemistry*
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Biochemistry / methods
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Buffers
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Chromatography, Affinity / instrumentation
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Chromatography, Affinity / methods*
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Hydrogen-Ion Concentration
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Proteins / chemistry
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Proteins / isolation & purification*
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Reducing Agents / chemistry
Substances
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Antibodies
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Buffers
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Proteins
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Reducing Agents