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SARS-CoV-2 neutralizing human recombinant antibodies selected from pre-pandemic healthy donors binding at RBD-ACE2 interface.
Bertoglio F, Meier D, Langreder N, Steinke S, Rand U, Simonelli L, Heine PA, Ballmann R, Schneider KT, Roth KDR, Ruschig M, Riese P, Eschke K, Kim Y, Schäckermann D, Pedotti M, Kuhn P, Zock-Emmenthal S, Wöhrle J, Kilb N, Herz T, Becker M, Grasshoff M, Wenzel EV, Russo G, Kröger A, Brunotte L, Ludwig S, Fühner V, Krämer SD, Dübel S, Varani L, Roth G, Čičin-Šain L, Schubert M, Hust M. Bertoglio F, et al. Among authors: steinke s. Nat Commun. 2021 Mar 11;12(1):1577. doi: 10.1038/s41467-021-21609-2. Nat Commun. 2021. PMID: 33707427 Free PMC article.
Baculovirus-free insect cell expression system for high yield antibody and antigen production.
Korn J, Schäckermann D, Kirmann T, Bertoglio F, Steinke S, Heisig J, Ruschig M, Rojas G, Langreder N, Wenzel EV, Roth KDR, Becker M, Meier D, van den Heuvel J, Hust M, Dübel S, Schubert M. Korn J, et al. Among authors: steinke s. Sci Rep. 2020 Dec 7;10(1):21393. doi: 10.1038/s41598-020-78425-9. Sci Rep. 2020. PMID: 33288836 Free PMC article.
A SARS-CoV-2 neutralizing antibody selected from COVID-19 patients binds to the ACE2-RBD interface and is tolerant to most known RBD mutations.
Bertoglio F, Fühner V, Ruschig M, Heine PA, Abassi L, Klünemann T, Rand U, Meier D, Langreder N, Steinke S, Ballmann R, Schneider KT, Roth KDR, Kuhn P, Riese P, Schäckermann D, Korn J, Koch A, Chaudhry MZ, Eschke K, Kim Y, Zock-Emmenthal S, Becker M, Scholz M, Moreira GMSG, Wenzel EV, Russo G, Garritsen HSP, Casu S, Gerstner A, Roth G, Adler J, Trimpert J, Hermann A, Schirrmann T, Dübel S, Frenzel A, Van den Heuvel J, Čičin-Šain L, Schubert M, Hust M. Bertoglio F, et al. Among authors: steinke s. Cell Rep. 2021 Jul 27;36(4):109433. doi: 10.1016/j.celrep.2021.109433. Epub 2021 Jul 7. Cell Rep. 2021. PMID: 34273271 Free PMC article.
Developing Recombinant Antibodies by Phage Display Against Infectious Diseases and Toxins for Diagnostics and Therapy.
Roth KDR, Wenzel EV, Ruschig M, Steinke S, Langreder N, Heine PA, Schneider KT, Ballmann R, Fühner V, Kuhn P, Schirrmann T, Frenzel A, Dübel S, Schubert M, Moreira GMSG, Bertoglio F, Russo G, Hust M. Roth KDR, et al. Among authors: steinke s. Front Cell Infect Microbiol. 2021 Jul 7;11:697876. doi: 10.3389/fcimb.2021.697876. eCollection 2021. Front Cell Infect Microbiol. 2021. PMID: 34307196 Free PMC article. Review.
Human serum from SARS-CoV-2-vaccinated and COVID-19 patients shows reduced binding to the RBD of SARS-CoV-2 Omicron variant.
Schubert M, Bertoglio F, Steinke S, Heine PA, Ynga-Durand MA, Maass H, Sammartino JC, Cassaniti I, Zuo F, Du L, Korn J, Milošević M, Wenzel EV, Krstanović F, Polten S, Pribanić-Matešić M, Brizić I, Baldanti F, Hammarström L, Dübel S, Šustić A, Marcotte H, Strengert M, Protić A, Piralla A, Pan-Hammarström Q, Čičin-Šain L, Hust M. Schubert M, et al. Among authors: steinke s. BMC Med. 2022 Mar 3;20(1):102. doi: 10.1186/s12916-022-02312-5. BMC Med. 2022. PMID: 35236358 Free PMC article.
NMDA-receptor-Fc-fusion constructs neutralize anti-NMDA receptor antibodies.
Steinke S, Kirmann T, Loi EA, Nerlich J, Weichard I, Kuhn P, Bullmann T, Ritzau-Jost A, Rizalar FS, Prüss H, Haucke V, Geis C, Hust M, Hallermann S. Steinke S, et al. Brain. 2023 May 2;146(5):1812-1820. doi: 10.1093/brain/awac497. Brain. 2023. PMID: 36866449 Free PMC article.
Construction of Human Immune and Naive scFv Phage Display Libraries.
Ruschig M, Heine PA, Fühner V, Zilkens KJK, Steinke S, Schubert M, Bertoglio F, Hust M. Ruschig M, et al. Among authors: steinke s. Methods Mol Biol. 2023;2702:15-37. doi: 10.1007/978-1-0716-3381-6_2. Methods Mol Biol. 2023. PMID: 37679613
Antibody Selection via Phage Display in Microtiter Plates.
Steinke S, Roth KDR, Ruschig M, Langreder N, Polten S, Schneider KT, Ballmann R, Russo G, Zilkens KJK, Schubert M, Bertoglio F, Hust M. Steinke S, et al. Methods Mol Biol. 2023;2702:247-260. doi: 10.1007/978-1-0716-3381-6_12. Methods Mol Biol. 2023. PMID: 37679623 Review.
Mapping Epitopes by Phage Display.
Steinke S, Roth KDR, Englick R, Langreder N, Ballmann R, Fühner V, Zilkens KJK, Moreira GMSG, Koch A, Azzali F, Russo G, Schubert M, Bertoglio F, Heine PA, Hust M. Steinke S, et al. Methods Mol Biol. 2023;2702:563-585. doi: 10.1007/978-1-0716-3381-6_28. Methods Mol Biol. 2023. PMID: 37679639
142 results