The most common and robust in vitro technology to generate monoclonal human antibodies is phage display. This technology is a widely used and powerful key technology for recombinant antibody selection. Phage display-derived antibodies are used as research tools, in diagnostic assays, and by 2022, 14 phage display-derived therapeutic antibodies were approved. In this review, we describe a fast high-throughput antibody (scFv) selection procedure in 96-well microtiter plates. The given detailed protocol allows the antibody selection ("panning"), screening, and identification of monoclonal antibodies in less than 2 weeks. Furthermore, we describe an on-rate panning approach for the selection of monoclonal antibodies with fast on-rates.
Keywords: Antibody fragments; Antibody phage display; Antibody selection; Microtiter plates (MTPs); Monoclonal antibodies; On-rate panning; Panning; Phage display; scFv.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.