Antibody phage display is a widely used in vitro selection technology for the generation of human recombinant antibodies and has yielded thousands of useful antibodies for research, diagnostics, and therapy. In order to successfully generate antibodies using phage display, the basis is the construction of high-quality antibody gene libraries. Here, we describe detailed methods for the construction of such high-quality immune and naive scFv gene libraries of human origin. These protocols were used to develop human naive (e.g., HAL9/10) and immune libraries, which resulted in thousands of specific antibodies for all kinds of applications.
Keywords: Antibody gene library; Antibody phage display; Immune antibody library; Library generation; Naive antibody library; Single-chain fragment variable (scFv).
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.