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Bump-and-hole engineering of human polypeptide N-acetylgalactosamine transferases to dissect their protein substrates and glycosylation sites in cells.
STAR Protoc. 2023 Mar 17;4(1):101974. doi: 10.1016/j.xpro.2022.101974. Epub 2023 Jan 11.
STAR Protoc. 2023.
PMID: 36633947
Free PMC article.
A photo-cross-linking GlcNAc analog enables covalent capture of N-linked glycoprotein-binding partners on the cell surface.
Wu H, Shajahan A, Yang JY, Capota E, Wands AM, Arthur CM, Stowell SR, Moremen KW, Azadi P, Kohler JJ.
Wu H, et al.
Cell Chem Biol. 2022 Jan 20;29(1):84-97.e8. doi: 10.1016/j.chembiol.2021.07.007. Epub 2021 Jul 30.
Cell Chem Biol. 2022.
PMID: 34331854
Free PMC article.
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Mass Spectrometric Method for the Unambiguous Profiling of Cellular Dynamic Glycosylation.
Shajahan A, Supekar NT, Wu H, Wands AM, Bhat G, Kalimurthy A, Matsubara M, Ranzinger R, Kohler JJ, Azadi P.
Shajahan A, et al.
ACS Chem Biol. 2020 Oct 16;15(10):2692-2701. doi: 10.1021/acschembio.0c00453. Epub 2020 Sep 4.
ACS Chem Biol. 2020.
PMID: 32809798
Free PMC article.
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Bump-and-Hole Engineering Identifies Specific Substrates of Glycosyltransferases in Living Cells.
Schumann B, Malaker SA, Wisnovsky SP, Debets MF, Agbay AJ, Fernandez D, Wagner LJS, Lin L, Li Z, Choi J, Fox DM, Peh J, Gray MA, Pedram K, Kohler JJ, Mrksich M, Bertozzi CR.
Schumann B, et al.
Mol Cell. 2020 Jun 4;78(5):824-834.e15. doi: 10.1016/j.molcel.2020.03.030. Epub 2020 Apr 22.
Mol Cell. 2020.
PMID: 32325029
Free PMC article.
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Photocrosslinking probes for capture of carbohydrate interactions.
Wu H, Kohler J.
Wu H, et al.
Curr Opin Chem Biol. 2019 Dec;53:173-182. doi: 10.1016/j.cbpa.2019.09.002. Epub 2019 Nov 6.
Curr Opin Chem Biol. 2019.
PMID: 31706134
Free PMC article.
Review.
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Structural basis of O-GlcNAc recognition by mammalian 14-3-3 proteins.
Toleman CA, Schumacher MA, Yu SH, Zeng W, Cox NJ, Smith TJ, Soderblom EJ, Wands AM, Kohler JJ, Boyce M.
Toleman CA, et al.
Proc Natl Acad Sci U S A. 2018 Jun 5;115(23):5956-5961. doi: 10.1073/pnas.1722437115. Epub 2018 May 21.
Proc Natl Acad Sci U S A. 2018.
PMID: 29784830
Free PMC article.
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A Conserved Splicing Silencer Dynamically Regulates O-GlcNAc Transferase Intron Retention and O-GlcNAc Homeostasis.
Park SK, Zhou X, Pendleton KE, Hunter OV, Kohler JJ, O'Donnell KA, Conrad NK.
Park SK, et al.
Cell Rep. 2017 Aug 1;20(5):1088-1099. doi: 10.1016/j.celrep.2017.07.017.
Cell Rep. 2017.
PMID: 28768194
Free PMC article.
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