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Advanced method for high-throughput expression of mutated eukaryotic membrane proteins in Saccharomyces cerevisiae.
Ito K, Sugawara T, Shiroishi M, Tokuda N, Kurokawa A, Misaka T, Makyio H, Yurugi-Kobayashi T, Shimamura T, Nomura N, Murata T, Abe K, Iwata S, Kobayashi T. Ito K, et al. Among authors: kobayashi t. Biochem Biophys Res Commun. 2008 Jul 11;371(4):841-5. doi: 10.1016/j.bbrc.2008.04.182. Epub 2008 May 12. Biochem Biophys Res Commun. 2008. PMID: 18474222
Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris.
Yurugi-Kobayashi T, Asada H, Shiroishi M, Shimamura T, Funamoto S, Katsuta N, Ito K, Sugawara T, Tokuda N, Tsujimoto H, Murata T, Nomura N, Haga K, Haga T, Iwata S, Kobayashi T. Yurugi-Kobayashi T, et al. Among authors: kobayashi t. Biochem Biophys Res Commun. 2009 Mar 6;380(2):271-6. doi: 10.1016/j.bbrc.2009.01.053. Epub 2009 Jan 22. Biochem Biophys Res Commun. 2009. PMID: 19167344
Crystallization and preliminary X-ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans.
Ito K, Ito S, Shimamura T, Kawarasaki Y, Abe K, Misaka T, Kobayashi T, Iwata S. Ito K, et al. Among authors: kobayashi t. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Sep 1;66(Pt 9):1086-8. doi: 10.1107/S1744309110029714. Epub 2010 Aug 28. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010. PMID: 20823533 Free PMC article.
Cysteine-to-serine shuffling using a Saccharomyces cerevisiae expression system improves protein secretion: case of a nonglycosylated mutant of miraculin, a taste-modifying protein.
Ito K, Sugawara T, Koizumi A, Nakajima K, Shimizu-Ibuka A, Shiroishi M, Asada H, Yurugi-Kobayashi T, Shimamura T, Asakura T, Misaka T, Iwata S, Kobayashi T, Abe K. Ito K, et al. Among authors: kobayashi t. Biotechnol Lett. 2011 Jan;33(1):103-7. doi: 10.1007/s10529-010-0399-1. Epub 2010 Oct 9. Biotechnol Lett. 2011. PMID: 20936326 Free article.
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