Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station "KIBO"

Hinako Hatae; Koji Inaka; Ryo Okamura; Naoki Furubayashi; Masayuki Kamo; Takuya Kobayashi; Yoshito Abe; So Iwata; Naotaka Hamasaki

doi:10.1016/j.ab.2018.08.009

Crystallization of Human Erythrocyte Band 3, the anion exchanger, at the International Space Station "KIBO"

Anal Biochem. 2018 Oct 15:559:91-93. doi: 10.1016/j.ab.2018.08.009. Epub 2018 Aug 15.

Authors

Hinako Hatae¹, Koji Inaka², Ryo Okamura³, Naoki Furubayashi², Masayuki Kamo², Takuya Kobayashi⁴, Yoshito Abe⁵, So Iwata⁴, Naotaka Hamasaki⁶

Affiliations

¹ Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan. Electronic address: hatae@niu.ac.jp.
² Maruwa Foods and Biosciences Inc., 170-1 Tsutsui-cho, Yamatokoriyama, Nara, 639-1123, Japan.
³ Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan.
⁴ Department of Medical Chemistry and Cell Biology, Kyoto University Faculty of Medicine, Konoe-cho, Yoshida, Sakyo-ku, Kyoto, 606-8501, Japan.
⁵ Department of Bio-Pharmaceutical Sciences, Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan.
⁶ Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki, 859-3298, Japan; Department of Clinical Chemistry and Laboratory Medicine, Kyushu University School of Medicine, Fukuoka, 812-8582, Japan. Electronic address: hamasaki@cclm.med.kyushu-u.ac.jp.

PMID: 30118660
DOI: 10.1016/j.ab.2018.08.009

Abstract

Band 3 mediates the Cl^- and HCO₃^- exchange across the red blood cell membrane and plays a pivotal role for delivering oxygen appropriately to metabolically active tissues. For understanding molecular mechanisms, it is essential to know the structure and function relationship. In terrestrial environments, however, nobody could make good quality crystals of Band 3 for the X-ray crystallographic study. In this study, we purified the transmembrane domain of Band 3 from human red blood cells and crystallized the purified Band 3 without the Fab fragment at the International Space Station "KIBO" under microgravity environments.

Keywords: Crystallization of band 3; Human erythrocyte; Multi-spanning membrane protein; The international space station "KIBO″; Under microgravity.

MeSH terms

Anion Exchange Protein 1, Erythrocyte / chemistry*
Anion Exchange Protein 1, Erythrocyte / isolation & purification*
Crystallization / methods*
Crystallography, X-Ray / methods
Erythrocyte Membrane / chemistry
Humans
Spacecraft*
Weightlessness*

Substances

Anion Exchange Protein 1, Erythrocyte