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Development of a high-throughput Gaussia luciferase reporter assay for the activation of the GADD45a gene by mutagens, promutagens, clastogens, and aneugens.
Hughes C, Rabinowitz A, Tate M, Birrell L, Allsup J, Billinton N, Walmsley RM. Hughes C, et al. J Biomol Screen. 2012 Dec;17(10):1302-15. doi: 10.1177/1087057112453312. Epub 2012 Jul 10. J Biomol Screen. 2012. PMID: 22786892
A protocol amendment also allows the assessment of pro-genotoxicity using S9 liver extracts. Compounds from the European Centre for the Validation of Alternative Methods (ECVAM) recommended list for the assessment of new or improved genotoxicity assays were evaluated with …
A protocol amendment also allows the assessment of pro-genotoxicity using S9 liver extracts. Compounds from the European Centre for t …
New and emerging technologies for genetic toxicity testing.
Lynch AM, Sasaki JC, Elespuru R, Jacobson-Kram D, Thybaud V, De Boeck M, Aardema MJ, Aubrecht J, Benz RD, Dertinger SD, Douglas GR, White PA, Escobar PA, Fornace A Jr, Honma M, Naven RT, Rusling JF, Schiestl RH, Walmsley RM, Yamamura E, van Benthem J, Kim JH. Lynch AM, et al. Environ Mol Mutagen. 2011 Apr;52(3):205-23. doi: 10.1002/em.20614. Epub 2010 Aug 25. Environ Mol Mutagen. 2011. PMID: 20740635 Review.
A pre-validation transferability study of the GreenScreen HC GADD45a-GFP assay with a metabolic activation system (S9).
Billinton N, Bruce S, Hansen JR, Hastwell PW, Jagger C, McComb C, Klug ML, Pant K, Rabinowitz A, Rees R, Tate M, Vinggaard AM, Walmsley RM. Billinton N, et al. Mutat Res. 2010 Jul 19;700(1-2):44-50. doi: 10.1016/j.mrgentox.2010.05.001. Epub 2010 May 7. Mutat Res. 2010. PMID: 20452458
In the spirit of assay validation by bodies such as the European Centre for the Validation of Alternative Methods (ECVAM), the adapted metabolic activation protocol for the GADD45a-GFP assay has been undergoing 'pre-validation'. ...
In the spirit of assay validation by bodies such as the European Centre for the Validation of Alternative Methods (ECVAM), the adapte …