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Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.
Sequeira AF, Turchetto J, Saez NJ, Peysson F, Ramond L, Duhoo Y, Blémont M, Fernandes VO, Gama LT, Ferreira LM, Guerreiro CI, Gilles N, Darbon H, Fontes CM, Vincentelli R. Sequeira AF, et al. Among authors: fontes cm. Microb Cell Fact. 2017 Jan 17;16(1):4. doi: 10.1186/s12934-016-0618-0. Microb Cell Fact. 2017. PMID: 28093085 Free PMC article.
High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.
Turchetto J, Sequeira AF, Ramond L, Peysson F, Brás JL, Saez NJ, Duhoo Y, Blémont M, Guerreiro CI, Quinton L, De Pauw E, Gilles N, Darbon H, Fontes CM, Vincentelli R. Turchetto J, et al. Among authors: fontes cm. Microb Cell Fact. 2017 Jan 17;16(1):6. doi: 10.1186/s12934-016-0617-1. Microb Cell Fact. 2017. PMID: 28095880 Free PMC article.
High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay.
Duhoo Y, Girault V, Turchetto J, Ramond L, Durbesson F, Fourquet P, Nominé Y, Cardoso V, Sequeira AF, Brás JLA, Fontes CMGA, Travé G, Wolff N, Vincentelli R. Duhoo Y, et al. Methods Mol Biol. 2019;2025:439-476. doi: 10.1007/978-1-4939-9624-7_21. Methods Mol Biol. 2019. PMID: 31267466
A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists.
Reynaud S, Ciolek J, Degueldre M, Saez NJ, Sequeira AF, Duhoo Y, Brás JLA, Meudal H, Cabo Díez M, Fernández Pedrosa V, Verdenaud M, Boeri J, Pereira Ramos O, Ducancel F, Vanden Driessche M, Fourmy R, Violette A, Upert G, Mourier G, Beck-Sickinger AG, Mörl K, Landon C, Fontes CMGA, Miñambres Herráiz R, Rodríguez de la Vega RC, Peigneur S, Tytgat J, Quinton L, De Pauw E, Vincentelli R, Servent D, Gilles N. Reynaud S, et al. J Med Chem. 2020 Aug 13;63(15):8250-8264. doi: 10.1021/acs.jmedchem.0c00485. Epub 2020 Jul 16. J Med Chem. 2020. PMID: 32602722
215 results