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Camelid nanobodies used as crystallization chaperones for different constructs of PorM, a component of the type IX secretion system from Porphyromonas gingivalis.
Duhoo Y, Roche J, Trinh TTN, Desmyter A, Gaubert A, Kellenberger C, Cambillau C, Roussel A, Leone P. Duhoo Y, et al. Acta Crystallogr F Struct Biol Commun. 2017 May 1;73(Pt 5):286-293. doi: 10.1107/S2053230X17005969. Epub 2017 Apr 26. Acta Crystallogr F Struct Biol Commun. 2017. PMID: 28471361 Free PMC article.
High-Throughput Production of a New Library of Human Single and Tandem PDZ Domains Allows Quantitative PDZ-Peptide Interaction Screening Through High-Throughput Holdup Assay.
Duhoo Y, Girault V, Turchetto J, Ramond L, Durbesson F, Fourquet P, Nominé Y, Cardoso V, Sequeira AF, Brás JLA, Fontes CMGA, Travé G, Wolff N, Vincentelli R. Duhoo Y, et al. Methods Mol Biol. 2019;2025:439-476. doi: 10.1007/978-1-4939-9624-7_21. Methods Mol Biol. 2019. PMID: 31267466
High-throughput expression of animal venom toxins in Escherichia coli to generate a large library of oxidized disulphide-reticulated peptides for drug discovery.
Turchetto J, Sequeira AF, Ramond L, Peysson F, Brás JL, Saez NJ, Duhoo Y, Blémont M, Guerreiro CI, Quinton L, De Pauw E, Gilles N, Darbon H, Fontes CM, Vincentelli R. Turchetto J, et al. Among authors: duhoo y. Microb Cell Fact. 2017 Jan 17;16(1):6. doi: 10.1186/s12934-016-0617-1. Microb Cell Fact. 2017. PMID: 28095880 Free PMC article.
Gene design, fusion technology and TEV cleavage conditions influence the purification of oxidized disulphide-rich venom peptides in Escherichia coli.
Sequeira AF, Turchetto J, Saez NJ, Peysson F, Ramond L, Duhoo Y, Blémont M, Fernandes VO, Gama LT, Ferreira LM, Guerreiro CI, Gilles N, Darbon H, Fontes CM, Vincentelli R. Sequeira AF, et al. Among authors: duhoo y. Microb Cell Fact. 2017 Jan 17;16(1):4. doi: 10.1186/s12934-016-0618-0. Microb Cell Fact. 2017. PMID: 28093085 Free PMC article.
A Venomics Approach Coupled to High-Throughput Toxin Production Strategies Identifies the First Venom-Derived Melanocortin Receptor Agonists.
Reynaud S, Ciolek J, Degueldre M, Saez NJ, Sequeira AF, Duhoo Y, Brás JLA, Meudal H, Cabo Díez M, Fernández Pedrosa V, Verdenaud M, Boeri J, Pereira Ramos O, Ducancel F, Vanden Driessche M, Fourmy R, Violette A, Upert G, Mourier G, Beck-Sickinger AG, Mörl K, Landon C, Fontes CMGA, Miñambres Herráiz R, Rodríguez de la Vega RC, Peigneur S, Tytgat J, Quinton L, De Pauw E, Vincentelli R, Servent D, Gilles N. Reynaud S, et al. Among authors: duhoo y. J Med Chem. 2020 Aug 13;63(15):8250-8264. doi: 10.1021/acs.jmedchem.0c00485. Epub 2020 Jul 16. J Med Chem. 2020. PMID: 32602722
Strategies for Heterologous Expression, Synthesis, and Purification of Animal Venom Toxins.
Rivera-de-Torre E, Rimbault C, Jenkins TP, Sørensen CV, Damsbo A, Saez NJ, Duhoo Y, Hackney CM, Ellgaard L, Laustsen AH. Rivera-de-Torre E, et al. Among authors: duhoo y. Front Bioeng Biotechnol. 2022 Jan 20;9:811905. doi: 10.3389/fbioe.2021.811905. eCollection 2021. Front Bioeng Biotechnol. 2022. PMID: 35127675 Free PMC article. Review.
The AAA+ ATPase RavA and its binding partner ViaA modulate E. coli aminoglycoside sensitivity through interaction with the inner membrane.
Felix J, Bumba L, Liesche C, Fraudeau A, Rébeillé F, El Khoury JY, Huard K, Gallet B, Moriscot C, Kleman JP, Duhoo Y, Jessop M, Kandiah E, Barras F, Jouhet J, Gutsche I. Felix J, et al. Among authors: duhoo y. Nat Commun. 2022 Sep 20;13(1):5502. doi: 10.1038/s41467-022-32992-9. Nat Commun. 2022. PMID: 36127320 Free PMC article.
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