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3,957 results

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Electrophoretic mobility shift assay (EMSA) for detecting protein-nucleic acid interactions.
Hellman LM, Fried MG. Hellman LM, et al. Nat Protoc. 2007;2(8):1849-61. doi: 10.1038/nprot.2007.249. Nat Protoc. 2007. PMID: 17703195 Free PMC article.
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. ...In this protocol, we identify the most important factors that determine the stabilities and electrophoretic mobilities of complexes un …
The gel electrophoresis mobility shift assay (EMSA) is used to detect protein complexes with nucleic acids. ...I …
Electrophoretic Mobility Shift Assays with GFP-Tagged Proteins (GFP-EMSA).
Sorenson AE, Schaeffer PM. Sorenson AE, et al. Methods Mol Biol. 2020;2089:159-166. doi: 10.1007/978-1-0716-0163-1_10. Methods Mol Biol. 2020. PMID: 31773653
The electrophoretic mobility shift assay (EMSA) is commonly used for the study of nucleic acid-binding proteins. The technique can be used to demonstrate that a protein is binding to RNA or DNA through visualization of a shift in elect …
The electrophoretic mobility shift assay (EMSA) is commonly used for the study of nucleic acid-binding pr …
Separation and Characterization of Protein-DNA Complexes by EMSA and In-Gel Footprinting.
Charlier D, Bervoets I. Charlier D, et al. Methods Mol Biol. 2022;2516:169-199. doi: 10.1007/978-1-0716-2413-5_11. Methods Mol Biol. 2022. PMID: 35922628
Hence, the technique combines the resolving power of protein-DNA complexes in the electrophoretic mobility shift assay (EMSA) with the precision of target site identification by chemical footprinting. ...
Hence, the technique combines the resolving power of protein-DNA complexes in the electrophoretic mobility shift ass …
Receptor-DNA interactions: EMSA and footprinting.
Read JT, Cheng H, Hendy SC, Nelson CC, Rennie PS. Read JT, et al. Methods Mol Biol. 2009;505:97-122. doi: 10.1007/978-1-60327-575-0_6. Methods Mol Biol. 2009. PMID: 19117141
RNA-RNA SELEX.
Cho B. Cho B. Methods Mol Biol. 2015;1240:39-47. doi: 10.1007/978-1-4939-1896-6_3. Methods Mol Biol. 2015. PMID: 25352135
RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA). Here, we describe the separation of the RNA aptamers binding to an RNA stem-loop target with affinity chromatography using the …
RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay ( …
The Interactome of Protein, DNA, and RNA.
Yan Y, Huang T. Yan Y, et al. Methods Mol Biol. 2023;2695:89-110. doi: 10.1007/978-1-0716-3346-5_6. Methods Mol Biol. 2023. PMID: 37450113 Review.
Electrophoretic Mobility Shift Assay (EMSA) and Microscale Thermophoresis (MST) Methods to Measure Interactions Between tRNAs and Their Modifying Enzymes.
Chramiec-Głąbik A, Rawski M, Glatt S, Lin TY. Chramiec-Głąbik A, et al. Methods Mol Biol. 2023;2666:29-53. doi: 10.1007/978-1-0716-3191-1_3. Methods Mol Biol. 2023. PMID: 37166655
In this chapter, we describe protocols to generate fluorescently labeled RNAs and outline the principles underlying electrophoretic mobility shift assays (EMSA) and microscale thermophoresis (MST). These two methods allow qualitative and quantitative e …
In this chapter, we describe protocols to generate fluorescently labeled RNAs and outline the principles underlying electrophoretic …
Southwestern Blotting Assay.
Jia Y, Nagore L, Jarrett H. Jia Y, et al. Methods Mol Biol. 2015;1334:85-99. doi: 10.1007/978-1-4939-2877-4_5. Methods Mol Biol. 2015. PMID: 26404144 Free PMC article.
To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electrophoresis, such as a gel shift (EMSA), can precede isoelectric focusing and SDS-PAGE to further improve separation. ...
To increase separation and determine isoelectric point a 2-dimensional gel can be blotted. Additional dimensions of electrophoresis, such as …
Fluorescein-Based Electrophoretic Mobility Shift Assay.
Mudiyanselage SDD, Wang Y. Mudiyanselage SDD, et al. Methods Mol Biol. 2022;2316:133-140. doi: 10.1007/978-1-0716-1464-8_12. Methods Mol Biol. 2022. PMID: 34845691
RNA-protein complexes are functionally important in biology. Electrophoretic mobility shift assays (EMSA) have been widely used to study the molecular basis of protein-RNA interactions. Previous methods for EMSA mostly relied on radioactive RNA …
RNA-protein complexes are functionally important in biology. Electrophoretic mobility shift assays (EMSA) have b …
Characterization of DNA-binding proteins using multiplexed competitor EMSA.
Smith AJ, Humphries SE. Smith AJ, et al. J Mol Biol. 2009 Jan 23;385(3):714-7. doi: 10.1016/j.jmb.2008.11.035. Epub 2008 Nov 27. J Mol Biol. 2009. PMID: 19059416
We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiplexed Competitor Electrophoretic Mobility Shift Assay, uses a series of multiplexed oligonucleotide DNA consensus …
We describe a low-cost high-throughput technique to characterize nuclear protein DNA-binding interactions. This technique, known as Multiple …
3,957 results