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Oocyte electroporation prior to in vitro fertilization is an efficient method to generate single, double, and multiple knockout porcine embryos of interest in biomedicine and animal production.
Navarro-Serna S, Piñeiro-Silva C, Fernández-Martín I, Dehesa-Etxebeste M, López de Munain A, Gadea J. Navarro-Serna S, et al. Among authors: pineiro silva c. Theriogenology. 2024 Apr 1;218:111-118. doi: 10.1016/j.theriogenology.2024.01.040. Epub 2024 Feb 1. Theriogenology. 2024. PMID: 38320372 Free article.
Generation of Calpain-3 knock-out porcine embryos by CRISPR-Cas9 electroporation and intracytoplasmic microinjection of oocytes before insemination.
Navarro-Serna S, Dehesa-Etxebeste M, Piñeiro-Silva C, Romar R, Lopes JS, López de Munaín A, Gadea J. Navarro-Serna S, et al. Among authors: pineiro silva c. Theriogenology. 2022 Jul 1;186:175-184. doi: 10.1016/j.theriogenology.2022.04.012. Epub 2022 Apr 22. Theriogenology. 2022. PMID: 35500431 Free article.