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Mutation of isoleucine 705 of the oxidosqualene-lanosterol cyclase from Saccharomyces cerevisiae affects lanosterol's C/D-ring cyclization and 17α/β-exocyclic side chain stereochemistry.
Org Biomol Chem. 2011 Feb 21;9(4):1092-7. doi: 10.1039/c0ob00582g. Epub 2010 Dec 15.
Org Biomol Chem. 2011.
PMID: 21157613
Importance of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase tyrosine 707 residue for chair-boat bicyclic ring formation and deprotonation reactions.
Wu TK, Wang TT, Chang CH, Liu YT, Shie WS.
Wu TK, et al. Among authors: shie ws.
Org Lett. 2008 Nov 6;10(21):4959-62. doi: 10.1021/ol802036c. Epub 2008 Oct 9.
Org Lett. 2008.
PMID: 18842050
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Alteration of the substrate's prefolded conformation and cyclization stereochemistry of oxidosqualene-lanosterol cyclase of Saccharomyces cerevisiae by substitution at phenylalanine 699.
Wu TK, Chang CH, Wen HY, Liu YT, Li WH, Wang TT, Shie WS.
Wu TK, et al. Among authors: shie ws.
Org Lett. 2010 Feb 5;12(3):500-3. doi: 10.1021/ol902694y.
Org Lett. 2010.
PMID: 20055456
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Protein engineering of Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase into parkeol synthase.
Liu YT, Hu TC, Chang CH, Shie WS, Wu TK.
Liu YT, et al. Among authors: shie ws.
Org Lett. 2012 Oct 19;14(20):5222-5. doi: 10.1021/ol302341h. Epub 2012 Oct 8.
Org Lett. 2012.
PMID: 23043506
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Protein engineering of oxidosqualene-lanosterol cyclase into triterpene monocyclase.
Chang CH, Wen HY, Shie WS, Lu CT, Li ME, Liu YT, Li WH, Wu TK.
Chang CH, et al. Among authors: shie ws.
Org Biomol Chem. 2013 Jul 7;11(25):4214-9. doi: 10.1039/c3ob40493e.
Org Biomol Chem. 2013.
PMID: 23680980
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