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Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of Pseudomonas cellulosa xylanase 10A.
Andrews SR, Charnock SJ, Lakey JH, Davies GJ, Claeyssens M, Nerinckx W, Underwood M, Sinnott ML, Warren RA, Gilbert HJ. Andrews SR, et al. Among authors: davies gj. J Biol Chem. 2000 Jul 28;275(30):23027-33. doi: 10.1074/jbc.M000128200. J Biol Chem. 2000. PMID: 10767281 Free article.
Substrate distortion by a beta-mannanase: snapshots of the Michaelis and covalent-intermediate complexes suggest a B(2,5) conformation for the transition state.
Ducros VM, Zechel DL, Murshudov GN, Gilbert HJ, Szabó L, Stoll D, Withers SG, Davies GJ. Ducros VM, et al. Among authors: davies gj. Angew Chem Int Ed Engl. 2002 Aug 2;41(15):2824-7. doi: 10.1002/1521-3773(20020802)41:15<2824::AID-ANIE2824>3.0.CO;2-G. Angew Chem Int Ed Engl. 2002. PMID: 12203498 No abstract available.
615 results