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Table representation of search results timeline featuring number of search results per year.

Year Number of Results
1949 1
1984 1
1992 2
1993 3
1994 2
1995 4
1996 3
1997 3
1998 3
1999 4
2000 4
2001 7
2002 1
2003 7
2004 10
2005 2
2006 14
2007 20
2008 18
2009 22
2010 36
2011 41
2012 53
2013 69
2014 90
2015 95
2016 103
2017 120
2018 145
2019 177
2020 228
2021 308
2022 365
2023 366
2024 176

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2,276 results

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Page 1
CRISPR-mediated modular RNA-guided regulation of transcription in eukaryotes.
Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, Weissman JS, Qi LS. Gilbert LA, et al. Cell. 2013 Jul 18;154(2):442-51. doi: 10.1016/j.cell.2013.06.044. Epub 2013 Jul 11. Cell. 2013. PMID: 23849981 Free PMC article.
The CRISPR-associated catalytically inactive dCas9 protein offers a general platform for RNA-guided DNA targeting. Here, we show that fusion of dCas9 to effector domains with distinct regulatory functions enables stable and efficient transcriptional repression or activatio …
The CRISPR-associated catalytically inactive dCas9 protein offers a general platform for RNA-guided DNA targeting. Here, we show that …
Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression.
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA. Qi LS, et al. Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. Cell. 2013. PMID: 23452860 Free PMC article.
Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. We show that a catalytically dead Cas9 lacking endonuclease activity, when coexpressed with a guide RNA, generates a DNA recognition …
Here, we develop a method for controlling gene expression based on Cas9, an RNA-guided DNA endonuclease from a type II CRISPR system. …
CRISPR/Cas9 in Genome Editing and Beyond.
Wang H, La Russa M, Qi LS. Wang H, et al. Annu Rev Biochem. 2016 Jun 2;85:227-64. doi: 10.1146/annurev-biochem-060815-014607. Epub 2016 Apr 25. Annu Rev Biochem. 2016. PMID: 27145843 Review.
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclea …
The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bact …
Manganese-Based Tumor Immunotherapy.
Zhang K, Qi C, Cai K. Zhang K, et al. Adv Mater. 2023 May;35(19):e2205409. doi: 10.1002/adma.202205409. Epub 2023 Mar 9. Adv Mater. 2023. PMID: 36121368 Review.
This comprehensive review focuses on the most recent progress of Mn-based nanoplatforms in tumor immunotherapy. The characteristics of Mn are first discussed to guide the design of Mn-based multifunctional nanoplatforms. Then the biomedical applications of Mn-based nanopla …
This comprehensive review focuses on the most recent progress of Mn-based nanoplatforms in tumor immunotherapy. The characteristics of Mn ar …
Engineered miniature CRISPR-Cas system for mammalian genome regulation and editing.
Xu X, Chemparathy A, Zeng L, Kempton HR, Shang S, Nakamura M, Qi LS. Xu X, et al. Mol Cell. 2021 Oct 21;81(20):4333-4345.e4. doi: 10.1016/j.molcel.2021.08.008. Epub 2021 Sep 3. Mol Cell. 2021. PMID: 34480847 Free article.
Here, we create an efficient miniature Cas system (CasMINI) engineered from the type V-F Cas12f (Cas14) system by guide RNA and protein engineering, which is less than half the size of currently used CRISPR systems (Cas9 or Cas12a). ...
Here, we create an efficient miniature Cas system (CasMINI) engineered from the type V-F Cas12f (Cas14) system by guide RNA and prote …
[Guiding-qi acupuncture for dry eye syndrome].
Xie W, Zeng L, Tao Y, Zhou Y, Zhao R, Huang X, Hou W, Zhang R, Zong L. Xie W, et al. Zhongguo Zhen Jiu. 2018 Feb 12;38(2):153-8. doi: 10.13703/j.0255-2930.2018.02.012. Zhongguo Zhen Jiu. 2018. PMID: 29473358 Clinical Trial. Chinese.
The control group was treated with conventional acupuncture, while the observation group was treated with guiding-qi acupuncture. Electroacupuncture (EA) was used at bilateral Tongziliao (GB1) and Cuanzhu (BL 2), 30 min per treatment. ...The total effective rate was …
The control group was treated with conventional acupuncture, while the observation group was treated with guiding-qi acupunctu …
A versatile CRISPR-Cas13d platform for multiplexed transcriptomic regulation and metabolic engineering in primary human T cells.
Tieu V, Sotillo E, Bjelajac JR, Chen C, Malipatlolla M, Guerrero JA, Xu P, Quinn PJ, Fisher C, Klysz D, Mackall CL, Qi LS. Tieu V, et al. Cell. 2024 Feb 29;187(5):1278-1295.e20. doi: 10.1016/j.cell.2024.01.035. Epub 2024 Feb 21. Cell. 2024. PMID: 38387457
To address these challenges, we developed multiplexed effector guide arrays (MEGA), a platform for programmable and scalable regulation of the T cell transcriptome using the RNA-guided, RNA-targeting activity of CRISPR-Cas13d. ...
To address these challenges, we developed multiplexed effector guide arrays (MEGA), a platform for programmable and scalable regulati …
[Discussion on "guiding meridian sinew qi along with meridian running course" in treatment of meridian sinew disorders].
Hu RJ, Jiang YL, Li RR, He XF, Fang JQ. Hu RJ, et al. Zhongguo Zhen Jiu. 2021 Mar 12;41(3):335-8. doi: 10.13703/j.0255-2930.20200215-k0004. Zhongguo Zhen Jiu. 2021. PMID: 33798321 Chinese.
It is to determine the effect mechanism and therapeutic method of the idea as "guiding meridian sinew qi along with meridian running course" for meridian sinew disorders so as to provide the reference for clinical treatment of these disorders. ...By investigating th …
It is to determine the effect mechanism and therapeutic method of the idea as "guiding meridian sinew qi along with meridian r …
[Professor LIANG Fan-rong's clinical application of the guiding-qi acupuncture technique].
Li DH, Liang H, Ren YL, Liang FR. Li DH, et al. Zhongguo Zhen Jiu. 2019 Jul 12;39(7):773-7. doi: 10.13703/j.0255-2930.2019.07.024. Zhongguo Zhen Jiu. 2019. PMID: 31286742 Chinese.
Firstly, the needle is inserted slowly so as to conduct the reversed turbid qi in yang back to yin. After arrival of qi, with the compound manipulation for promoting qi, qi is guided to the affected site. When the treatment ends, the needle is r …
Firstly, the needle is inserted slowly so as to conduct the reversed turbid qi in yang back to yin. After arrival of qi, with …
2,276 results