Sequence-specific Ni(II)-dependent peptide bond hydrolysis for protein engineering. Combinatorial library determination of optimal sequences.
Krezel A, Kopera E, Protas AM, Poznański J, Wysłouch-Cieszyńska A, Bal W.
Krezel A, et al.
J Am Chem Soc. 2010 Mar 17;132(10):3355-66. doi: 10.1021/ja907567r.
J Am Chem Soc. 2010.
PMID: 20166730
Previously we demonstrated for several examples that peptides having a general internal sequence R(N)-Yaa-Ser/Thr-Xaa-His-Zaa-R(C) (Yaa = Glu or Ala, Xaa = Ala or His, Zaa = Lys, R(N) and R(C) = any N- and C-terminal amino acid sequence) were hydrolyzed specifically at the …
Previously we demonstrated for several examples that peptides having a general internal sequence R(N)-Yaa-Ser/Thr-Xaa-His-Zaa-R(C) (Y …