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Characterization of a beta-fructofuranosidase from Schwanniomyces occidentalis with transfructosylating activity yielding the prebiotic 6-kestose.
Alvaro-Benito M, de Abreu M, Fernández-Arrojo L, Plou FJ, Jiménez-Barbero J, Ballesteros A, Polaina J, Fernández-Lobato M. Alvaro-Benito M, et al. Among authors: fernandez lobato m, fernandez arrojo l. J Biotechnol. 2007 Oct 15;132(1):75-81. doi: 10.1016/j.jbiotec.2007.07.939. Epub 2007 Jul 26. J Biotechnol. 2007. PMID: 17904238
New insights into the fructosyltransferase activity of Schwanniomyces occidentalis ß-fructofuranosidase, emerging from nonconventional codon usage and directed mutation.
Alvaro-Benito M, de Abreu M, Portillo F, Sanz-Aparicio J, Fernández-Lobato M. Alvaro-Benito M, et al. Among authors: fernandez lobato m. Appl Environ Microbiol. 2010 Nov;76(22):7491-9. doi: 10.1128/AEM.01614-10. Epub 2010 Sep 17. Appl Environ Microbiol. 2010. PMID: 20851958 Free PMC article.
Production of Galacto-oligosaccharides by the β-Galactosidase from Kluyveromyces lactis : comparative analysis of permeabilized cells versus soluble enzyme.
Rodriguez-Colinas B, de Abreu MA, Fernandez-Arrojo L, de Beer R, Poveda A, Jimenez-Barbero J, Haltrich D, Ballesteros Olmo AO, Fernandez-Lobato M, Plou FJ. Rodriguez-Colinas B, et al. Among authors: fernandez lobato m, fernandez arrojo l. J Agric Food Chem. 2011 Oct 12;59(19):10477-84. doi: 10.1021/jf2022012. Epub 2011 Sep 8. J Agric Food Chem. 2011. PMID: 21888310
Structural and kinetic insights reveal that the amino acid pair Gln-228/Asn-254 modulates the transfructosylating specificity of Schwanniomyces occidentalis β-fructofuranosidase, an enzyme that produces prebiotics.
Álvaro-Benito M, Sainz-Polo MA, González-Pérez D, González B, Plou FJ, Fernández-Lobato M, Sanz-Aparicio J. Álvaro-Benito M, et al. Among authors: fernandez lobato m. J Biol Chem. 2012 Jun 1;287(23):19674-86. doi: 10.1074/jbc.M112.355503. Epub 2012 Apr 16. J Biol Chem. 2012. PMID: 22511773 Free PMC article.
58 results