Abstract
The extracellular β-fructofuranosidase Xd-INV from the yeast Xanthophyllomyces dendrorhous mainly synthesizes the neo-fructooligosaccharides (neo-FOS) neokestose and neonystose. This enzyme is a glycoprotein with a content of 59-67% N-linked carbohydrates and an estimated molecular mass of 160-200 kDa. The extent level of glycosylation affects the thermal behaviour of the enzyme but not its hydrolase and transferase activities, which are optimal at 60-70 °C. The neo-FOS yield of this enzyme was increased from 40 to 168 g/L when the sucrose concentration increased from 420 to 600 g/L and when the reaction was carried out at 60 °C. The neo-FOS levels obtained (168 g/L) in this work are the largest reported for any microbial β-fructofuranosidase.
Copyright © 2012 Elsevier Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Basidiomycota / enzymology*
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Biocatalysis
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Chromatography, High Pressure Liquid
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Electrophoresis, Polyacrylamide Gel
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Extracellular Space / enzymology*
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Glycosylation
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Hydrolases / metabolism
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Kinetics
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Oligosaccharides / biosynthesis*
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Oligosaccharides / chemistry
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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase / metabolism
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Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Sucrose / analysis
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Sucrose / chemistry
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Temperature
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Time Factors
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Transferases / metabolism
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Trisaccharides / biosynthesis
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Trisaccharides / chemistry
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beta-Fructofuranosidase / metabolism*
Substances
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Oligosaccharides
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Trisaccharides
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fructooligosaccharide
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neokestose
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Sucrose
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Transferases
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Hydrolases
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beta-Fructofuranosidase
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Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase