Porkka EJ - Search Results

1

Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli.

Hytönen VP, Laitinen OH, Airenne TT, Kidron H, Meltola NJ, Porkka EJ, Hörhä J, Paldanius T, Määttä JA, Nordlund HR, Johnson MS, Salminen TA, Airenne KJ, Ylä-Herttuala S, Kulomaa MS. Hytönen VP, et al. Among authors: porkka ej. Biochem J. 2004 Dec 1;384(Pt 2):385-90. doi: 10.1042/BJ20041114. Biochem J. 2004. PMID: 15324300 Free PMC article.

2

Chicken avidin-related protein 4/5 shows superior thermal stability when compared with avidin while retaining high affinity to biotin.

Hytönen VP, Nyholm TK, Pentikäinen OT, Vaarno J, Porkka EJ, Nordlund HR, Johnson MS, Slotte JP, Laitinen OH, Kulomaa MS. Hytönen VP, et al. Among authors: porkka ej. J Biol Chem. 2004 Mar 5;279(10):9337-43. doi: 10.1074/jbc.M310989200. Epub 2003 Dec 3. J Biol Chem. 2004. PMID: 14660583 Free article.

3

Tetravalent single-chain avidin: from subunits to protein domains via circularly permuted avidins.

Nordlund HR, Hytönen VP, Hörhä J, Määttä JA, White DJ, Halling K, Porkka EJ, Slotte JP, Laitinen OH, Kulomaa MS. Nordlund HR, et al. Among authors: porkka ej. Biochem J. 2005 Dec 15;392(Pt 3):485-91. doi: 10.1042/BJ20051038. Biochem J. 2005. PMID: 16092919 Free PMC article.

4

Dual-affinity avidin molecules.

Hytönen VP, Nordlund HR, Hörhä J, Nyholm TK, Hyre DE, Kulomaa T, Porkka EJ, Marttila AT, Stayton PS, Laitinen OH, Kulomaa MS. Hytönen VP, et al. Among authors: porkka ej. Proteins. 2005 Nov 15;61(3):597-607. doi: 10.1002/prot.20604. Proteins. 2005. PMID: 16175628

5

Chicken avidin-related proteins show altered biotin-binding and physico-chemical properties as compared with avidin.

Laitinen OH, Hytönen VP, Ahlroth MK, Pentikäinen OT, Gallagher C, Nordlund HR, Ovod V, Marttila AT, Porkka E, Heino S, Johnson MS, Airenne KJ, Kulomaa MS. Laitinen OH, et al. Biochem J. 2002 May 1;363(Pt 3):609-17. doi: 10.1042/0264-6021:3630609. Biochem J. 2002. PMID: 11964162 Free PMC article.

6

Introduction of histidine residues into avidin subunit interfaces allows pH-dependent regulation of quaternary structure and biotin binding.

Nordlund HR, Hytönen VP, Laitinen OH, Uotila ST, Niskanen EA, Savolainen J, Porkka E, Kulomaa MS. Nordlund HR, et al. FEBS Lett. 2003 Dec 18;555(3):449-54. doi: 10.1016/s0014-5793(03)01302-4. FEBS Lett. 2003. PMID: 14675754 Free article.

7

Construction of a dual chain pseudotetrameric chicken avidin by combining two circularly permuted avidins.

Nordlund HR, Laitinen OH, Hytönen VP, Uotila ST, Porkka E, Kulomaa MS. Nordlund HR, et al. J Biol Chem. 2004 Aug 27;279(35):36715-9. doi: 10.1074/jbc.M403496200. Epub 2004 May 6. J Biol Chem. 2004. PMID: 15131113 Free article.

8

Design and construction of highly stable, protease-resistant chimeric avidins.

Hytönen VP, Määttä JA, Nyholm TK, Livnah O, Eisenberg-Domovich Y, Hyre D, Nordlund HR, Hörhä J, Niskanen EA, Paldanius T, Kulomaa T, Porkka EJ, Stayton PS, Laitinen OH, Kulomaa MS. Hytönen VP, et al. Among authors: porkka ej. J Biol Chem. 2005 Mar 18;280(11):10228-33. doi: 10.1074/jbc.M414196200. Epub 2005 Jan 13. J Biol Chem. 2005. PMID: 15649900 Free article.

9

Mutation of a critical tryptophan to lysine in avidin or streptavidin may explain why sea urchin fibropellin adopts an avidin-like domain.

Laitinen OH, Airenne KJ, Marttila AT, Kulik T, Porkka E, Bayer EA, Wilchek M, Kulomaa MS. Laitinen OH, et al. FEBS Lett. 1999 Nov 12;461(1-2):52-8. doi: 10.1016/s0014-5793(99)01423-4. FEBS Lett. 1999. PMID: 10561495 Free article.

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