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Adenine base-editing-mediated exon skipping induces gene knockout in cultured pig cells.
Zhu XX, Pan JS, Lin T, Yang YC, Huang QY, Yang SP, Qu ZX, Lin ZS, Wen JC, Yan AF, Feng J, Liu L, Zhang XL, Lu JH, Tang DS. Zhu XX, et al. Biotechnol Lett. 2022 Jan;44(1):59-76. doi: 10.1007/s10529-021-03214-x. Epub 2022 Jan 8. Biotechnol Lett. 2022. PMID: 34997407
Gene-knockout pigs have important applications in agriculture and medicine. Compared with CRISPR/Cas9, Adenine base editor (ABE) convert single A.T pairs to G.C pairs in the genome without generating DNA double-strand breaks, and this method has higher accuracy and biosafe …
Gene-knockout pigs have important applications in agriculture and medicine. Compared with CRISPR/Cas9, Adenine base editor (ABE) conv …
Application of the modified cytosine base-editing in the cultured cells of bama minipig.
Pan JS, Lin ZS, Wen JC, Guo JF, Wu XH, Liu YY, Lai WJ, Liang QY, Xie YS, Chen YR, Chen YH, Yan AF, Feng J, Liu L, Gong DY, Zhu XX, Lu JH, Tang DS. Pan JS, et al. Biotechnol Lett. 2021 Sep;43(9):1699-1714. doi: 10.1007/s10529-021-03159-1. Epub 2021 Jun 30. Biotechnol Lett. 2021. PMID: 34189671
Unfavorable characteristics are their low lean meat percentage, high fat content, slow growth rate, and low feed conversion ratio. Genome-editing technology using CRISPR/Cas9 efficiently knocked out the myostatin gene (MSTN) that has a negative regulatory effect on muscle …
Unfavorable characteristics are their low lean meat percentage, high fat content, slow growth rate, and low feed conversion ratio. Genome-ed …