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High sensitive one-step RT-PCR using MMLV reverse transcriptase, DNA polymerase with reverse transcriptase activity, and DNA/RNA helicase.
Okano H, Baba M, Yamasaki T, Hidese R, Fujiwara S, Yanagihara I, Ujiiye T, Hayashi T, Kojima K, Takita T, Yasukawa K. Okano H, et al. Among authors: yanagihara i. Biochem Biophys Res Commun. 2017 May 20;487(1):128-133. doi: 10.1016/j.bbrc.2017.04.030. Epub 2017 Apr 8. Biochem Biophys Res Commun. 2017. PMID: 28400277 No abstract available.
Next-generation sequencing-based analysis of reverse transcriptase fidelity.
Yasukawa K, Iida K, Okano H, Hidese R, Baba M, Yanagihara I, Kojima K, Takita T, Fujiwara S. Yasukawa K, et al. Among authors: yanagihara i. Biochem Biophys Res Commun. 2017 Oct 14;492(2):147-153. doi: 10.1016/j.bbrc.2017.07.169. Epub 2017 Aug 1. Biochem Biophys Res Commun. 2017. PMID: 28778390
Thermostable DNA helicase improves the sensitivity of digital PCR.
Hidese R, Kawato K, Nakura Y, Fujiwara A, Yasukawa K, Yanagihara I, Fujiwara S. Hidese R, et al. Among authors: yanagihara i. Biochem Biophys Res Commun. 2018 Jan 15;495(3):2189-2194. doi: 10.1016/j.bbrc.2017.12.053. Epub 2017 Dec 9. Biochem Biophys Res Commun. 2018. PMID: 29233693
Type II restriction modification system in Ureaplasma parvum OMC-P162 strain.
Wu HN, Nakura Y, Yoshimura M, Gaddi Tantengco OA, Nomiyama M, Takayanagi T, Fujita T, Yasukawa K, Yanagihara I. Wu HN, et al. Among authors: yanagihara i. PLoS One. 2018 Oct 16;13(10):e0205328. doi: 10.1371/journal.pone.0205328. eCollection 2018. PLoS One. 2018. PMID: 30325937 Free PMC article.
121 results