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A general protocol for the generation of Nanobodies for structural biology.
Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J. Pardon E, et al. Among authors: wohlkonig a. Nat Protoc. 2014 Mar;9(3):674-93. doi: 10.1038/nprot.2014.039. Epub 2014 Feb 27. Nat Protoc. 2014. PMID: 24577359 Free PMC article.
Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM.
Uchański T, Masiulis S, Fischer B, Kalichuk V, López-Sánchez U, Zarkadas E, Weckener M, Sente A, Ward P, Wohlkönig A, Zögg T, Remaut H, Naismith JH, Nury H, Vranken W, Aricescu AR, Pardon E, Steyaert J. Uchański T, et al. Among authors: wohlkonig a. Nat Methods. 2021 Jan;18(1):60-68. doi: 10.1038/s41592-020-01001-6. Epub 2021 Jan 6. Nat Methods. 2021. PMID: 33408403 Free PMC article.
Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein.
Abskharon RN, Soror SH, Pardon E, El Hassan H, Legname G, Steyaert J, Wohlkonig A. Abskharon RN, et al. Among authors: wohlkonig a. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1644-6. doi: 10.1107/S1744309110042168. Epub 2010 Nov 26. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010. PMID: 21139215 Free PMC article.
A novel expression system for production of soluble prion proteins in E. coli.
Abskharon RN, Ramboarina S, El Hassan H, Gad W, Apostol MI, Giachin G, Legname G, Steyaert J, Messens J, Soror SH, Wohlkonig A. Abskharon RN, et al. Among authors: wohlkonig a. Microb Cell Fact. 2012 Jan 10;11:6. doi: 10.1186/1475-2859-11-6. Microb Cell Fact. 2012. PMID: 22233534 Free PMC article.
43 results