Villegas EN - Search Results

1

The Applicability of TaqMan-Based Quantitative Real-Time PCR Assays for Detecting and Enumerating Cryptosporidium spp. Oocysts in the Environment.

Staggs SE, Beckman EM, Keely SP, Mackwan R, Ware MW, Moyer AP, Ferretti JA, Sayed A, Xiao L, Villegas EN. Staggs SE, et al. Among authors: villegas en. PLoS One. 2013 Jun 21;8(6):e66562. doi: 10.1371/journal.pone.0066562. Print 2013. PLoS One. 2013. PMID: 23805235 Free PMC article.

2

Development and evaluation of an off-the-slide genotyping technique for identifying Giardia cysts and Cryptosporidium oocysts directly from US EPA Method 1623 slides.

Ware MW, Keely SP, Villegas EN. Ware MW, et al. Among authors: villegas en. J Appl Microbiol. 2013 Jul;115(1):298-309. doi: 10.1111/jam.12223. Epub 2013 May 15. J Appl Microbiol. 2013. PMID: 23594204 Free article.

3

Cryptosporidium propidium monoazide-PCR, a molecular biology-based technique for genotyping of viable Cryptosporidium oocysts.

Brescia CC, Griffin SM, Ware MW, Varughese EA, Egorov AI, Villegas EN. Brescia CC, et al. Among authors: villegas en. Appl Environ Microbiol. 2009 Nov;75(21):6856-63. doi: 10.1128/AEM.00540-09. Epub 2009 Sep 11. Appl Environ Microbiol. 2009. PMID: 19749067 Free PMC article.

4

Improved Cryptosporidium parvum oocyst propagation using dexamethasone suppressed CF-1 mice.

Ware MW, Villegas EN. Ware MW, et al. Among authors: villegas en. Vet Parasitol. 2010 Mar 25;168(3-4):329-31. doi: 10.1016/j.vetpar.2009.11.019. Epub 2009 Dec 1. Vet Parasitol. 2010. PMID: 20036060

5

Evaluation of four RNA extraction methods for gene expression analyses of Cryptosporidium parvum and Toxoplasma gondii oocysts.

See MJ, Staggs SE, Dubey JP, Villegas EN. See MJ, et al. Among authors: villegas en. J Microbiol Methods. 2012 Jun;89(3):185-92. doi: 10.1016/j.mimet.2012.03.010. Epub 2012 Mar 15. J Microbiol Methods. 2012. PMID: 22465221

6

The development and implementation of a method using blue mussels (Mytilus spp.) as biosentinels of Cryptosporidium spp. and Toxoplasma gondii contamination in marine aquatic environments.

Staggs SE, Keely SP, Ware MW, Schable N, See MJ, Gregorio D, Zou X, Su C, Dubey JP, Villegas EN. Staggs SE, et al. Among authors: villegas en. Parasitol Res. 2015 Dec;114(12):4655-67. doi: 10.1007/s00436-015-4711-9. Epub 2015 Sep 11. Parasitol Res. 2015. PMID: 26358104

7

Differences in staining intensities affect reported occurrences and concentrations of Giardia spp. in surface drinking water sources.

Alderisio KA, Villegas LF, Ware MW, McDonald LA, Xiao L, Villegas EN. Alderisio KA, et al. Among authors: villegas lf, villegas en. J Appl Microbiol. 2017 Dec;123(6):1607-1613. doi: 10.1111/jam.13585. Epub 2017 Oct 22. J Appl Microbiol. 2017. PMID: 28910512 Free PMC article.

8

Using quantitative reverse transcriptase PCR and cell culture plaque assays to determine resistance of Toxoplasma gondii oocysts to chemical sanitizers.

Villegas EN, Augustine SA, Villegas LF, Ware MW, See MJ, Lindquist HD, Schaefer FW 3rd, Dubey JP. Villegas EN, et al. Among authors: villegas lf. J Microbiol Methods. 2010 Jun;81(3):219-25. doi: 10.1016/j.mimet.2010.03.023. Epub 2010 Apr 10. J Microbiol Methods. 2010. PMID: 20385175

9

Determining UV inactivation of Toxoplasma gondii oocysts by using cell culture and a mouse bioassay.

Ware MW, Augustine SA, Erisman DO, See MJ, Wymer L, Hayes SL, Dubey JP, Villegas EN. Ware MW, et al. Among authors: villegas en. Appl Environ Microbiol. 2010 Aug;76(15):5140-7. doi: 10.1128/AEM.00153-10. Epub 2010 Jun 11. Appl Environ Microbiol. 2010. PMID: 20543052 Free PMC article.

10

Application of leftover sample material from waterborne protozoa monitoring for the molecular detection of Bacteroidales and fecal source tracking markers.

Ryu H, Tran H, Ware MW, Iker B, Griffin S, Egorov A, Edge TA, Newmann N, Villegas EN, Domingo JW. Ryu H, et al. Among authors: villegas en. J Microbiol Methods. 2011 Sep;86(3):337-43. doi: 10.1016/j.mimet.2011.06.001. Epub 2011 Jun 13. J Microbiol Methods. 2011. PMID: 21693138

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