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Development of an intermediate chromatography step in an insulin purification process. The use of a High Throughput Process Development approach based on selectivity parameters.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Dec 15;973C:126-132. doi: 10.1016/j.jchromb.2014.09.024. Epub 2014 Sep 28.
J Chromatogr B Analyt Technol Biomed Life Sci. 2014.
PMID: 25464105
Purification of bovine pancreatic glucagon as a by-product of insulin production.
Andrade AS, Vilela L, Tunes H.
Andrade AS, et al. Among authors: tunes h.
Braz J Med Biol Res. 1997 Dec;30(12):1421-6. doi: 10.1590/s0100-879x1997001200006.
Braz J Med Biol Res. 1997.
PMID: 9686160
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Recombinant human insulin IX. Investigation of factors, influencing the folding of fusion protein-S-sulfonates, biotechnological precursors of human insulin.
Tikhonov RV, Pechenov SE, Belacheu IA, Yakimov SA, Klyushnichenko VE, Tunes H, Thiemann JE, Vilela L, Wulfson AN.
Tikhonov RV, et al. Among authors: tunes h.
Protein Expr Purif. 2002 Nov;26(2):187-93. doi: 10.1016/s1046-5928(02)00531-4.
Protein Expr Purif. 2002.
PMID: 12406671
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Recombinant human insulin. VIII. Isolation of fusion protein--S-sulfonate, biotechnological precursor of human insulin, from the biomass of transformed Escherichia coli cells.
Tikhonov RV, Pechenov SE, Belacheu IA, Yakimov SA, Klyushnichenko VE, Boldireva EF, Korobko VG, Tunes H, Thiemann JE, Vilela L, Wulfson AN.
Tikhonov RV, et al. Among authors: tunes h.
Protein Expr Purif. 2001 Feb;21(1):176-82. doi: 10.1006/prep.2000.1345.
Protein Expr Purif. 2001.
PMID: 11162404
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Alpha- and beta- rat urinary kallikreins: chemical and physicochemical properties.
Tunes H, Silva E, Mares-Guia M.
Tunes H, et al.
Braz J Med Biol Res. 1983 Oct;16(3):193-202.
Braz J Med Biol Res. 1983.
PMID: 6557824
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Alpha and beta rat urinary kallikreins: chemical and physicochemical properties.
Tunes H, Silva E, Mares-Guia M.
Tunes H, et al.
Adv Exp Med Biol. 1983;156:317-27.
Adv Exp Med Biol. 1983.
PMID: 6552843
No abstract available.
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Competitive inhibition by the D-isomer in racemic mixtures used as substrate in kinetic studies: a simple method for data treatment.
Mares-Guia M, Silva E, Tunes H.
Mares-Guia M, et al. Among authors: tunes h.
Arch Biochem Biophys. 1984 Jan;228(1):278-81. doi: 10.1016/0003-9861(84)90068-7.
Arch Biochem Biophys. 1984.
PMID: 6320735
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