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Novel suspension retroviral packaging cells generated by transposition using transposase encoding mRNA advance vector yields and enable production in bioreactors.
Front Bioeng Biotechnol. 2023 Apr 4;11:1076524. doi: 10.3389/fbioe.2023.1076524. eCollection 2023.
Front Bioeng Biotechnol. 2023.
PMID: 37082212
Free PMC article.
Transgene Expression and Transposition Efficiency of Two-Component Sleeping Beauty Transposon Vector Systems Utilizing Plasmid or mRNA Encoding the Transposase.
Tschorn N, van Heuvel Y, Stitz J.
Tschorn N, et al.
Mol Biotechnol. 2023 Aug;65(8):1327-1335. doi: 10.1007/s12033-022-00642-6. Epub 2022 Dec 22.
Mol Biotechnol. 2023.
PMID: 36547824
Free PMC article.
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Transposon vector-mediated stable gene transfer for the accelerated establishment of recombinant mammalian cell pools allowing for high-yield production of biologics.
Tschorn N, Berg K, Stitz J.
Tschorn N, et al.
Biotechnol Lett. 2020 Jul;42(7):1103-1112. doi: 10.1007/s10529-020-02889-y. Epub 2020 Apr 22.
Biotechnol Lett. 2020.
PMID: 32323079
Free PMC article.
Review.
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Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Genotype-Phenotype Coupling Transposon Vectors.
Berg K, Schäfer VN, Tschorn N, Stitz J.
Berg K, et al. Among authors: tschorn n.
Methods Mol Biol. 2020;2070:351-361. doi: 10.1007/978-1-4939-9853-1_20.
Methods Mol Biol. 2020.
PMID: 31625106
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Ecotropic HIV-1 vectors pseudotyped with R-peptide-deleted envelope protein variants reveal improved gene transfer efficiencies.
Tschorn N, Söhngen C, Berg K, Stitz J.
Tschorn N, et al.
Virology. 2022 Dec;577:124-130. doi: 10.1016/j.virol.2022.09.008. Epub 2022 Oct 25.
Virology. 2022.
PMID: 36343471
Free article.
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