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A general protocol for the generation of Nanobodies for structural biology.
Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J. Pardon E, et al. Among authors: triest s. Nat Protoc. 2014 Mar;9(3):674-93. doi: 10.1038/nprot.2014.039. Epub 2014 Feb 27. Nat Protoc. 2014. PMID: 24577359 Free PMC article.
Structure of an endosomal signaling GPCR-G protein-β-arrestin megacomplex.
Nguyen AH, Thomsen ARB, Cahill TJ 3rd, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little J 4th, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ. Nguyen AH, et al. Among authors: triest s. Nat Struct Mol Biol. 2019 Dec;26(12):1123-1131. doi: 10.1038/s41594-019-0330-y. Epub 2019 Nov 18. Nat Struct Mol Biol. 2019. PMID: 31740855 Free PMC article.
Distinct conformations of GPCR-β-arrestin complexes mediate desensitization, signaling, and endocytosis.
Cahill TJ 3rd, Thomsen AR, Tarrasch JT, Plouffe B, Nguyen AH, Yang F, Huang LY, Kahsai AW, Bassoni DL, Gavino BJ, Lamerdin JE, Triest S, Shukla AK, Berger B, Little J 4th, Antar A, Blanc A, Qu CX, Chen X, Kawakami K, Inoue A, Aoki J, Steyaert J, Sun JP, Bouvier M, Skiniotis G, Lefkowitz RJ. Cahill TJ 3rd, et al. Among authors: triest s. Proc Natl Acad Sci U S A. 2017 Mar 7;114(10):2562-2567. doi: 10.1073/pnas.1701529114. Epub 2017 Feb 21. Proc Natl Acad Sci U S A. 2017. PMID: 28223524 Free PMC article.
Accelerating GPCR Drug Discovery With Conformation-Stabilizing VHHs.
Laeremans T, Sands ZA, Claes P, De Blieck A, De Cesco S, Triest S, Busch A, Felix D, Kumar A, Jaakola VP, Menet C. Laeremans T, et al. Among authors: triest s. Front Mol Biosci. 2022 May 23;9:863099. doi: 10.3389/fmolb.2022.863099. eCollection 2022. Front Mol Biosci. 2022. PMID: 35677880 Free PMC article. Review.
15 results