Steyaert J - Search Results

1

A general protocol for the generation of Nanobodies for structural biology.

Pardon E, Laeremans T, Triest S, Rasmussen SG, Wohlkönig A, Ruf A, Muyldermans S, Hol WG, Kobilka BK, Steyaert J. Pardon E, et al. Among authors: steyaert j. Nat Protoc. 2014 Mar;9(3):674-93. doi: 10.1038/nprot.2014.039. Epub 2014 Feb 27. Nat Protoc. 2014. PMID: 24577359 Free PMC article.

2

Nanobody-aided structure determination of the EpsI:EpsJ pseudopilin heterodimer from Vibrio vulnificus.

Lam AY, Pardon E, Korotkov KV, Hol WGJ, Steyaert J. Lam AY, et al. Among authors: steyaert j. J Struct Biol. 2009 Apr;166(1):8-15. doi: 10.1016/j.jsb.2008.11.008. Epub 2008 Dec 10. J Struct Biol. 2009. PMID: 19118632 Free PMC article.

3

Crystal structure of the N-terminal domain of the secretin GspD from ETEC determined with the assistance of a nanobody.

Korotkov KV, Pardon E, Steyaert J, Hol WG. Korotkov KV, et al. Among authors: steyaert j. Structure. 2009 Feb 13;17(2):255-65. doi: 10.1016/j.str.2008.11.011. Structure. 2009. PMID: 19217396 Free PMC article.

4

Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris.

Ryckaert S, Pardon E, Steyaert J, Callewaert N. Ryckaert S, et al. Among authors: steyaert j. J Biotechnol. 2010 Jan 15;145(2):93-8. doi: 10.1016/j.jbiotec.2009.10.010. Epub 2009 Oct 25. J Biotechnol. 2010. PMID: 19861136

5

An intrabody based on a llama single-domain antibody targeting the N-terminal alpha-helical multimerization domain of HIV-1 rev prevents viral production.

Vercruysse T, Pardon E, Vanstreels E, Steyaert J, Daelemans D. Vercruysse T, et al. Among authors: steyaert j. J Biol Chem. 2010 Jul 9;285(28):21768-80. doi: 10.1074/jbc.M110.112490. Epub 2010 Apr 20. J Biol Chem. 2010. PMID: 20406803 Free PMC article.

6

Structures of a key interaction protein from the Trypanosoma brucei editosome in complex with single domain antibodies.

Wu M, Park YJ, Pardon E, Turley S, Hayhurst A, Deng J, Steyaert J, Hol WG. Wu M, et al. Among authors: steyaert j. J Struct Biol. 2011 Apr;174(1):124-36. doi: 10.1016/j.jsb.2010.10.007. Epub 2010 Oct 20. J Struct Biol. 2011. PMID: 20969962 Free PMC article.

7

Crystallization and preliminary X-ray diffraction analysis of a specific VHH domain against mouse prion protein.

Abskharon RN, Soror SH, Pardon E, El Hassan H, Legname G, Steyaert J, Wohlkonig A. Abskharon RN, et al. Among authors: steyaert j. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010 Dec 1;66(Pt 12):1644-6. doi: 10.1107/S1744309110042168. Epub 2010 Nov 26. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010. PMID: 21139215 Free PMC article.

8

Atomic structure of a nanobody-trapped domain-swapped dimer of an amyloidogenic beta2-microglobulin variant.

Domanska K, Vanderhaegen S, Srinivasan V, Pardon E, Dupeux F, Marquez JA, Giorgetti S, Stoppini M, Wyns L, Bellotti V, Steyaert J. Domanska K, et al. Among authors: steyaert j. Proc Natl Acad Sci U S A. 2011 Jan 25;108(4):1314-9. doi: 10.1073/pnas.1008560108. Epub 2011 Jan 10. Proc Natl Acad Sci U S A. 2011. PMID: 21220305 Free PMC article.

9

Structure of a nanobody-stabilized active state of the β(2) adrenoceptor.

Rasmussen SG, Choi HJ, Fung JJ, Pardon E, Casarosa P, Chae PS, Devree BT, Rosenbaum DM, Thian FS, Kobilka TS, Schnapp A, Konetzki I, Sunahara RK, Gellman SH, Pautsch A, Steyaert J, Weis WI, Kobilka BK. Rasmussen SG, et al. Among authors: steyaert j. Nature. 2011 Jan 13;469(7329):175-80. doi: 10.1038/nature09648. Nature. 2011. PMID: 21228869 Free PMC article.

10

Measuring cooperative Rev protein-protein interactions on Rev responsive RNA by fluorescence resonance energy transfer.

Vercruysse T, Pawar S, De Borggraeve W, Pardon E, Pavlakis GN, Pannecouque C, Steyaert J, Balzarini J, Daelemans D. Vercruysse T, et al. Among authors: steyaert j. RNA Biol. 2011 Mar-Apr;8(2):316-24. doi: 10.4161/rna.8.2.13782. Epub 2011 Mar 1. RNA Biol. 2011. PMID: 21358282 Free PMC article.

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