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Base editing enables duplex point mutagenesis in Clostridium autoethanogenum at the price of numerous off-target mutations.
Front Bioeng Biotechnol. 2023 Jul 10;11:1211197. doi: 10.3389/fbioe.2023.1211197. eCollection 2023.
Front Bioeng Biotechnol. 2023.
PMID: 37496853
Free PMC article.
A Gold Standard, CRISPR/Cas9-Based Complementation Strategy Reliant on 24 Nucleotide Bookmark Sequences.
Seys FM, Rowe P, Bolt EL, Humphreys CM, Minton NP.
Seys FM, et al.
Genes (Basel). 2020 Apr 23;11(4):458. doi: 10.3390/genes11040458.
Genes (Basel). 2020.
PMID: 32340238
Free PMC article.
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CRISPR-Cas9D10A nickase-assisted base editing in the solvent producer Clostridium beijerinckii.
Li Q, Seys FM, Minton NP, Yang J, Jiang Y, Jiang W, Yang S.
Li Q, et al. Among authors: seys fm.
Biotechnol Bioeng. 2019 Jun;116(6):1475-1483. doi: 10.1002/bit.26949. Epub 2019 Feb 21.
Biotechnol Bioeng. 2019.
PMID: 30739328
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