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Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α.
Synth Syst Biotechnol. 2024 Mar 27;9(3):416-424. doi: 10.1016/j.synbio.2024.03.011. eCollection 2024 Sep.
Synth Syst Biotechnol. 2024.
PMID: 38601208
Free PMC article.
A Cost-Effective Pichia pastoris Cell-Free System Driven by Glycolytic Intermediates Enables the Production of Complex Eukaryotic Proteins.
Schloßhauer JL, Dondapati SK, Kubick S, Zemella A.
Schloßhauer JL, et al.
Bioengineering (Basel). 2024 Jan 18;11(1):92. doi: 10.3390/bioengineering11010092.
Bioengineering (Basel). 2024.
PMID: 38247969
Free PMC article.
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Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool.
Schloßhauer JL, Zemella A, Dondapati SK, Thoring L, Meyer M, Kubick S.
Schloßhauer JL, et al.
Sci Rep. 2023 Sep 14;13(1):15236. doi: 10.1038/s41598-023-42198-8.
Sci Rep. 2023.
PMID: 37709815
Free PMC article.
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Just Keep Rolling?-An Encompassing Review towards Accelerated Vaccine Product Life Cycles.
Stiefel J, Zimmer J, Schloßhauer JL, Vosen A, Kilz S, Balakin S.
Stiefel J, et al. Among authors: schlosshauer jl.
Vaccines (Basel). 2023 Jul 27;11(8):1287. doi: 10.3390/vaccines11081287.
Vaccines (Basel). 2023.
PMID: 37631855
Free PMC article.
Review.
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One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycol.
Pouyan P, Zemella A, Schloßhauer JL, Walter RM, Haag R, Kubick S.
Pouyan P, et al. Among authors: schlosshauer jl.
Sci Rep. 2023 Apr 19;13(1):6394. doi: 10.1038/s41598-023-33463-x.
Sci Rep. 2023.
PMID: 37076514
Free PMC article.
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Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems.
Schloßhauer JL, Cavak N, Zemella A, Thoring L, Kubick S.
Schloßhauer JL, et al.
Front Mol Biosci. 2022 Apr 14;9:832379. doi: 10.3389/fmolb.2022.832379. eCollection 2022.
Front Mol Biosci. 2022.
PMID: 35586195
Free PMC article.
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Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications.
Ramm F, Jack L, Kaser D, Schloßhauer JL, Zemella A, Kubick S.
Ramm F, et al. Among authors: schlosshauer jl.
Toxins (Basel). 2022 Mar 23;14(4):233. doi: 10.3390/toxins14040233.
Toxins (Basel). 2022.
PMID: 35448842
Free PMC article.
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