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Plant-fed versus chemicals-fed rhizobacteria of Lucerne: Plant-only teabags culture media not only increase culturability of rhizobacteria but also recover a previously uncultured Lysobacter sp., Novosphingobium sp. and Pedobacter sp.
Hegazi NA, Sarhan MS, Fayez M, Patz S, Murphy BR, Ruppel S. Hegazi NA, et al. Among authors: sarhan ms. PLoS One. 2017 Jul 7;12(7):e0180424. doi: 10.1371/journal.pone.0180424. eCollection 2017. PLoS One. 2017. PMID: 28686606 Free PMC article.
Plant Materials are Sustainable Substrates Supporting New Technologies of Plant-Only-Based Culture Media for in vitro Culturing of the Plant Microbiota.
Mourad EF, Sarhan MS, Daanaa HA, Abdou M, Morsi AT, Abdelfadeel MR, Elsawey H, Nemr R, El-Tahan M, Hamza MA, Abbas M, Youssef HH, Abdelhadi AA, Amer WM, Fayez M, Ruppel S, Hegazi NA. Mourad EF, et al. Among authors: sarhan ms. Microbes Environ. 2018 Mar 29;33(1):40-49. doi: 10.1264/jsme2.ME17135. Epub 2018 Mar 23. Microbes Environ. 2018. PMID: 29479006 Free PMC article.
G3 PhyloChip Analysis Confirms the Promise of Plant-Based Culture Media for Unlocking the Composition and Diversity of the Maize Root Microbiome and for Recovering Unculturable Candidate Divisions/Phyla.
Sarhan MS, Patz S, Hamza MA, Youssef HH, Mourad EF, Fayez M, Murphy B, Ruppel S, Hegazi NA. Sarhan MS, et al. Microbes Environ. 2018 Sep 29;33(3):317-325. doi: 10.1264/jsme2.ME18023. Epub 2018 Sep 12. Microbes Environ. 2018. PMID: 30210099 Free PMC article.
An inoculum-dependent culturing strategy (IDC) for the cultivation of environmental microbiomes and the isolation of novel endophytic Actinobacteria.
Sarhan MS, Mourad EF, Nemr RA, Abdelfadeel MR, Daanaa HA, Youssef HH, Goda HA, Hamza MA, Fayez M, Eichler-Löbermann B, Ruppel S, Hegazi NA. Sarhan MS, et al. J Antibiot (Tokyo). 2020 Jan;73(1):66-71. doi: 10.1038/s41429-019-0226-4. Epub 2019 Aug 29. J Antibiot (Tokyo). 2020. PMID: 31467444 Free PMC article.
"In situ similis" Culturing of Plant Microbiota: A Novel Simulated Environmental Method Based on Plant Leaf Blades as Nutritional Pads.
Nemr RA, Khalil M, Sarhan MS, Abbas M, Elsawey H, Youssef HH, Hamza MA, Morsi AT, El-Tahan M, Fayez M, Patz S, Witzel K, Ruppel S, El-Sahhar KF, Hegazi NA. Nemr RA, et al. Among authors: sarhan ms. Front Microbiol. 2020 Apr 7;11:454. doi: 10.3389/fmicb.2020.00454. eCollection 2020. Front Microbiol. 2020. PMID: 32318031 Free PMC article.
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