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Methods for competitive enrichment and evaluation of superior DNA ligases.
Methods Enzymol. 2020;644:209-225. doi: 10.1016/bs.mie.2020.04.061. Epub 2020 May 20.
Methods Enzymol. 2020.
PMID: 32943146
A functional screen for recovery of 4'-phosphopantetheinyl transferase and associated natural product biosynthesis genes from metagenome libraries.
Owen JG, Robins KJ, Parachin NS, Ackerley DF.
Owen JG, et al. Among authors: robins kj.
Environ Microbiol. 2012 May;14(5):1198-209. doi: 10.1111/j.1462-2920.2012.02699.x. Epub 2012 Feb 22.
Environ Microbiol. 2012.
PMID: 22356582
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Escherichia coli NemA is an efficient chromate reductase that can be biologically immobilized to provide a cell free system for remediation of hexavalent chromium.
Robins KJ, Hooks DO, Rehm BH, Ackerley DF.
Robins KJ, et al.
PLoS One. 2013;8(3):e59200. doi: 10.1371/journal.pone.0059200. Epub 2013 Mar 13.
PLoS One. 2013.
PMID: 23527133
Free PMC article.
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Generating Functional Recombinant NRPS Enzymes in the Laboratory Setting via Peptidyl Carrier Protein Engineering.
Owen JG, Calcott MJ, Robins KJ, Ackerley DF.
Owen JG, et al. Among authors: robins kj.
Cell Chem Biol. 2016 Nov 17;23(11):1395-1406. doi: 10.1016/j.chembiol.2016.09.014. Epub 2016 Oct 27.
Cell Chem Biol. 2016.
PMID: 27984027
Free article.
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A sensitive single-enzyme assay system using the non-ribosomal peptide synthetase BpsA for measurement of L-glutamine in biological samples.
Brown AS, Robins KJ, Ackerley DF.
Brown AS, et al. Among authors: robins kj.
Sci Rep. 2017 Jan 31;7:41745. doi: 10.1038/srep41745.
Sci Rep. 2017.
PMID: 28139746
Free PMC article.
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Intracellular complexities of acquiring a new enzymatic function revealed by mass-randomisation of active-site residues.
Hall KR, Robins KJ, Williams EM, Rich MH, Calcott MJ, Copp JN, Little RF, Schwörer R, Evans GB, Patrick WM, Ackerley DF.
Hall KR, et al. Among authors: robins kj.
Elife. 2020 Nov 13;9:e59081. doi: 10.7554/eLife.59081.
Elife. 2020.
PMID: 33185191
Free PMC article.
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Development of a compartmentalised self-replication protocol for selection of superior blunt-end DNA ligases.
Stevenson LJ, Robins KJ, Sharma JK, Williams EM, Sharrock AV, Williamson AK, Arcus VL, Patrick WM, Ackerley DF.
Stevenson LJ, et al. Among authors: robins kj.
Enzyme Microb Technol. 2023 Feb;163:110153. doi: 10.1016/j.enzmictec.2022.110153. Epub 2022 Nov 5.
Enzyme Microb Technol. 2023.
PMID: 36403327
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Gene editing enables rapid engineering of complex antibiotic assembly lines.
Thong WL, Zhang Y, Zhuo Y, Robins KJ, Fyans JK, Herbert AJ, Law BJC, Micklefield J.
Thong WL, et al. Among authors: robins kj.
Nat Commun. 2021 Nov 25;12(1):6872. doi: 10.1038/s41467-021-27139-1.
Nat Commun. 2021.
PMID: 34824225
Free PMC article.
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