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CRISPR-Cas12a exhibits metal-dependent specificity switching.
Nguyen GT, Schelling MA, Buscher KA, Sritharan A, Sashital DG. Nguyen GT, et al. bioRxiv [Preprint]. 2024 Jan 17:2023.11.29.569287. doi: 10.1101/2023.11.29.569287. bioRxiv. 2024. PMID: 38076861 Free PMC article. Preprint.
A tool for more specific DNA integration.
Dhingra Y, Sashital DG. Dhingra Y, et al. Science. 2023 Nov 17;382(6672):768-769. doi: 10.1126/science.adl0863. Epub 2023 Nov 16. Science. 2023. PMID: 37972178
CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes.
Adler BA, Trinidad MI, Bellieny-Rabelo D, Zhang E, Karp HM, Skopintsev P, Thornton BW, Weissman RF, Yoon PH, Chen L, Hessler T, Eggers AR, Colognori D, Boger R, Doherty EE, Tsuchida CA, Tran RV, Hofman L, Shi H, Wasko KM, Zhou Z, Xia C, Al-Shimary MJ, Patel JR, Thomas VCJX, Pattali R, Kan MJ, Vardapetyan A, Yang A, Lahiri A, Maxwell MF, Murdock AG, Ramit GC, Henderson HR, Calvert RW, Bamert RS, Knott GJ, Lapinaite A, Pausch P, Cofsky JC, Sontheimer EJ, Wiedenheft B, Fineran PC, Brouns SJJ, Sashital DG, Thomas BC, Brown CT, Goltsman DSA, Barrangou R, Siksnys V, Banfield JF, Savage DF, Doudna JA. Adler BA, et al. Nucleic Acids Res. 2024 Jan 5;52(D1):D590-D596. doi: 10.1093/nar/gkad890. Nucleic Acids Res. 2024. PMID: 37889041 Free PMC article.