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Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth.
Gydush G, Nguyen E, Bae JH, Blewett T, Rhoades J, Reed SC, Shea D, Xiong K, Liu R, Yu F, Leong KW, Choudhury AD, Stover DG, Tolaney SM, Krop IE, Christopher Love J, Parsons HA, Mike Makrigiorgos G, Golub TR, Adalsteinsson VA. Gydush G, et al. Nat Biomed Eng. 2022 Mar;6(3):257-266. doi: 10.1038/s41551-022-00855-9. Epub 2022 Mar 17. Nat Biomed Eng. 2022. PMID: 35301450 Free PMC article.
Nuclease-Assisted Minor Allele Enrichment Using Overlapping Probes-Assisted Amplification-Refractory Mutation System: An Approach for the Improvement of Amplification-Refractory Mutation System-Polymerase Chain Reaction Specificity in Liquid Biopsies.
Markou A, Tzanikou E, Ladas I, Makrigiorgos GM, Lianidou E. Markou A, et al. Anal Chem. 2019 Oct 15;91(20):13105-13111. doi: 10.1021/acs.analchem.9b03325. Epub 2019 Oct 3. Anal Chem. 2019. PMID: 31538770 Free PMC article.
Denaturation-Enhanced Droplet Digital PCR for Liquid Biopsies.
Fitarelli-Kiehl M, Yu F, Ashtaputre R, Leong KW, Ladas I, Supplee J, Paweletz C, Mitra D, Schoenfeld JD, Parangi S, Makrigiorgos GM. Fitarelli-Kiehl M, et al. Clin Chem. 2018 Dec;64(12):1762-1771. doi: 10.1373/clinchem.2018.293845. Epub 2018 Oct 1. Clin Chem. 2018. PMID: 30274976 Free PMC article.