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Optimization and revision of the production process of the Necator americanus glutathione S-transferase 1 (Na-GST-1), the lead hookworm vaccine recombinant protein candidate.
Curti E, Seid CA, Hudspeth E, Center L, Rezende W, Pollet J, Kwityn C, Hammond M, Matsunami RK, Engler DA, Hotez PJ, Elena Bottazzi M. Curti E, et al. Among authors: pollet j. Hum Vaccin Immunother. 2014;10(7):1914-25. doi: 10.4161/hv.28872. Hum Vaccin Immunother. 2014. PMID: 25424799 Free PMC article.
Expression, purification, and characterization of the Necator americanus aspartic protease-1 (Na-APR-1 (M74)) antigen, a component of the bivalent human hookworm vaccine.
Seid CA, Curti E, Jones RM, Hudspeth E, Rezende W, Pollet J, Center L, Versteeg L, Pritchard S, Musiychuk K, Yusibov V, Hotez PJ, Bottazzi ME. Seid CA, et al. Among authors: pollet j. Hum Vaccin Immunother. 2015;11(6):1474-88. doi: 10.1080/21645515.2015.1036207. Hum Vaccin Immunother. 2015. PMID: 25905574 Free PMC article.
Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties.
Hudspeth EM, Wang Q, Seid CA, Hammond M, Wei J, Liu Z, Zhan B, Pollet J, Heffernan MJ, McAtee CP, Engler DA, Matsunami RK, Strych U, Asojo OA, Hotez PJ, Bottazzi ME. Hudspeth EM, et al. Among authors: pollet j. Hum Vaccin Immunother. 2016 Jul 2;12(7):1707-20. doi: 10.1080/21645515.2016.1139254. Epub 2016 Feb 2. Hum Vaccin Immunother. 2016. PMID: 26839079 Free PMC article.
Cysteine mutagenesis improves the production without abrogating antigenicity of a recombinant protein vaccine candidate for human chagas disease.
Seid CA, Jones KM, Pollet J, Keegan B, Hudspeth E, Hammond M, Wei J, McAtee CP, Versteeg L, Gutierrez A, Liu Z, Zhan B, Respress JL, Strych U, Bottazzi ME, Hotez PJ. Seid CA, et al. Among authors: pollet j. Hum Vaccin Immunother. 2017 Mar 4;13(3):621-633. doi: 10.1080/21645515.2016.1242540. Epub 2016 Oct 13. Hum Vaccin Immunother. 2017. PMID: 27737611 Free PMC article.
Structure of SALO, a leishmaniasis vaccine candidate from the sand fly Lutzomyia longipalpis.
Asojo OA, Kelleher A, Liu Z, Pollet J, Hudspeth EM, Rezende WC, Groen MJ, Seid CA, Abdeladhim M, Townsend S, de Castro W, Mendes-Sousa A, Bartholomeu DC, Fujiwara RT, Bottazzi ME, Hotez PJ, Zhan B, Oliveira F, Kamhawi S, Valenzuela JG. Asojo OA, et al. Among authors: pollet j. PLoS Negl Trop Dis. 2017 Mar 9;11(3):e0005374. doi: 10.1371/journal.pntd.0005374. eCollection 2017 Mar. PLoS Negl Trop Dis. 2017. PMID: 28278244 Free PMC article.
Optimization of the Production Process and Characterization of the Yeast-Expressed SARS-CoV Recombinant Receptor-Binding Domain (RBD219-N1), a SARS Vaccine Candidate.
Chen WH, Chag SM, Poongavanam MV, Biter AB, Ewere EA, Rezende W, Seid CA, Hudspeth EM, Pollet J, McAtee CP, Strych U, Bottazzi ME, Hotez PJ. Chen WH, et al. Among authors: pollet j. J Pharm Sci. 2017 Aug;106(8):1961-1970. doi: 10.1016/j.xphs.2017.04.037. Epub 2017 Apr 26. J Pharm Sci. 2017. PMID: 28456726 Free PMC article.
212 results