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Robust production of recombinant phosphoproteins using cell-free protein synthesis.
Nat Commun. 2015 Sep 9;6:8168. doi: 10.1038/ncomms9168.
Nat Commun. 2015.
PMID: 26350765
Free PMC article.
A flexible codon in genomically recoded Escherichia coli permits programmable protein phosphorylation.
Pirman NL, Barber KW, Aerni HR, Ma NJ, Haimovich AD, Rogulina S, Isaacs FJ, Rinehart J.
Pirman NL, et al.
Nat Commun. 2015 Sep 9;6:8130. doi: 10.1038/ncomms9130.
Nat Commun. 2015.
PMID: 26350500
Free PMC article.
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Phosphorylated WNK kinase networks in recoded bacteria recapitulate physiological function.
Schiapparelli P, Pirman NL, Mohler K, Miranda-Herrera PA, Zarco N, Kilic O, Miller C, Shah SR, Rogulina S, Hungerford W, Abriola L, Hoyer D, Turk BE, Guerrero-Cázares H, Isaacs FJ, Quiñones-Hinojosa A, Levchenko A, Rinehart J.
Schiapparelli P, et al. Among authors: pirman nl.
Cell Rep. 2021 Jul 20;36(3):109416. doi: 10.1016/j.celrep.2021.109416.
Cell Rep. 2021.
PMID: 34289367
Free PMC article.
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Comprehensive profiling of the STE20 kinase family defines features essential for selective substrate targeting and signaling output.
Miller CJ, Lou HJ, Simpson C, van de Kooij B, Ha BH, Fisher OS, Pirman NL, Boggon TJ, Rinehart J, Yaffe MB, Linding R, Turk BE.
Miller CJ, et al. Among authors: pirman nl.
PLoS Biol. 2019 Mar 21;17(3):e2006540. doi: 10.1371/journal.pbio.2006540. eCollection 2019 Mar.
PLoS Biol. 2019.
PMID: 30897078
Free PMC article.
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Continuous wave W- and D-band EPR spectroscopy offer "sweet-spots" for characterizing conformational changes and dynamics in intrinsically disordered proteins.
Casey TM, Liu Z, Esquiaqui JM, Pirman NL, Milshteyn E, Fanucci GE.
Casey TM, et al. Among authors: pirman nl.
Biochem Biophys Res Commun. 2014 Jul 18;450(1):723-8. doi: 10.1016/j.bbrc.2014.06.045. Epub 2014 Jun 17.
Biochem Biophys Res Commun. 2014.
PMID: 24950408
Free PMC article.
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Characterization of the disordered-to-α-helical transition of IA₃ by SDSL-EPR spectroscopy.
Pirman NL, Milshteyn E, Galiano L, Hewlett JC, Fanucci GE.
Pirman NL, et al.
Protein Sci. 2011 Jan;20(1):150-9. doi: 10.1002/pro.547.
Protein Sci. 2011.
PMID: 21080428
Free PMC article.
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