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A comparison of phosphospecific affinity reagents reveals the utility of recombinant Forkhead-associated domains in recognizing phosphothreonine-containing peptides.
N Biotechnol. 2016 Sep 25;33(5 Pt A):537-43. doi: 10.1016/j.nbt.2015.12.006. Epub 2016 Jan 6.
N Biotechnol. 2016.
PMID: 26772725
Free PMC article.
Directed evolution of the forkhead-associated domain to generate anti-phosphospecific reagents by phage display.
Pershad K, Wypisniak K, Kay BK.
Pershad K, et al.
J Mol Biol. 2012 Nov 23;424(1-2):88-103. doi: 10.1016/j.jmb.2012.09.006. Epub 2012 Sep 15.
J Mol Biol. 2012.
PMID: 22985966
Free PMC article.
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Drop-out phagemid vector for switching from phage displayed affinity reagents to expression formats.
Pershad K, Sullivan MA, Kay BK.
Pershad K, et al.
Anal Biochem. 2011 May 15;412(2):210-6. doi: 10.1016/j.ab.2011.02.006. Epub 2011 Mar 1.
Anal Biochem. 2011.
PMID: 21315061
Free PMC article.
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Generating thermal stable variants of protein domains through phage display.
Pershad K, Kay BK.
Pershad K, et al.
Methods. 2013 Mar 15;60(1):38-45. doi: 10.1016/j.ymeth.2012.12.009. Epub 2012 Dec 29.
Methods. 2013.
PMID: 23276752
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Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display.
Pershad K, Pavlovic JD, Gräslund S, Nilsson P, Colwill K, Karatt-Vellatt A, Schofield DJ, Dyson MR, Pawson T, Kay BK, McCafferty J.
Pershad K, et al.
Protein Eng Des Sel. 2010 Apr;23(4):279-88. doi: 10.1093/protein/gzq003. Epub 2010 Feb 17.
Protein Eng Des Sel. 2010.
PMID: 20164216
Free PMC article.
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Mapping protein interactions by combining antibody affinity maturation and mass spectrometry.
Dyson MR, Zheng Y, Zhang C, Colwill K, Pershad K, Kay BK, Pawson T, McCafferty J.
Dyson MR, et al. Among authors: pershad k.
Anal Biochem. 2011 Oct 1;417(1):25-35. doi: 10.1016/j.ab.2011.05.005. Epub 2011 May 31.
Anal Biochem. 2011.
PMID: 21704603
Free PMC article.
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A roadmap to generate renewable protein binders to the human proteome.
Colwill K; Renewable Protein Binder Working Group; Gräslund S.
Colwill K, et al.
Nat Methods. 2011 May 15;8(7):551-8. doi: 10.1038/nmeth.1607.
Nat Methods. 2011.
PMID: 21572409
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