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Precipitation with polyethylene glycol followed by washing and pelleting by ultracentrifugation enriches extracellular vesicles from tissue culture supernatants in small and large scales.
Ludwig AK, De Miroschedji K, Doeppner TR, Börger V, Ruesing J, Rebmann V, Durst S, Jansen S, Bremer M, Behrmann E, Singer BB, Jastrow H, Kuhlmann JD, El Magraoui F, Meyer HE, Hermann DM, Opalka B, Raunser S, Epple M, Horn PA, Giebel B. Ludwig AK, et al. Among authors: opalka b. J Extracell Vesicles. 2018 Oct 17;7(1):1528109. doi: 10.1080/20013078.2018.1528109. eCollection 2018. J Extracell Vesicles. 2018. PMID: 30357008 Free PMC article.
Gfi1b: a key player in the genesis and maintenance of acute myeloid leukemia and myelodysplastic syndrome.
Thivakaran A, Botezatu L, Hönes JM, Schütte J, Vassen L, Al-Matary YS, Patnana P, Zeller A, Heuser M, Thol F, Gabdoulline R, Olberding N, Frank D, Suslo M, Köster R, Lennartz K, Görgens A, Giebel B, Opalka B, Dührsen U, Khandanpour C. Thivakaran A, et al. Among authors: opalka b. Haematologica. 2018 Apr;103(4):614-625. doi: 10.3324/haematol.2017.167288. Epub 2018 Jan 11. Haematologica. 2018. PMID: 29326122 Free PMC article.
Expansion of functional personalized cells with specific transgene combinations.
Lipps C, Klein F, Wahlicht T, Seiffert V, Butueva M, Zauers J, Truschel T, Luckner M, Köster M, MacLeod R, Pezoldt J, Hühn J, Yuan Q, Müller PP, Kempf H, Zweigerdt R, Dittrich-Breiholz O, Pufe T, Beckmann R, Drescher W, Riancho J, Sañudo C, Korff T, Opalka B, Rebmann V, Göthert JR, Alves PM, Ott M, Schucht R, Hauser H, Wirth D, May T. Lipps C, et al. Among authors: opalka b. Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4. Nat Commun. 2018. PMID: 29520052 Free PMC article.
Thalidomide in combination with dexamethasone for pretreated patients with multiple myeloma: serum level of soluble interleukin-2 receptor as a predictive factor for response rate and for survival.
Schütt P, Ebeling P, Buttkereit U, Brandhorst D, Opalka B, Poser M, Müller S, Flasshove M, Moritz T, Seeber S, Nowrousian MR. Schütt P, et al. Among authors: opalka b. Ann Hematol. 2005 Sep;84(9):594-600. doi: 10.1007/s00277-005-1007-7. Epub 2005 Mar 3. Ann Hematol. 2005. PMID: 15744524 Clinical Trial.
In vitro dendritic cell generation and lymphocyte subsets in myeloma patients: influence of thalidomide and high-dose chemotherapy treatment.
Schütt P, Buttkereit U, Brandhorst D, Lindemann M, Schmiedl S, Grosse-Wilde H, Seeber S, Nowrousian MR, Opalka B, Moritz T. Schütt P, et al. Among authors: opalka b. Cancer Immunol Immunother. 2005 May;54(5):506-12. doi: 10.1007/s00262-004-0633-6. Epub 2004 Nov 20. Cancer Immunol Immunother. 2005. PMID: 15750834 Free PMC article.
106 results