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Limiting factors in Escherichia coli fed-batch production of recombinant proteins.
Sandén AM, Prytz I, Tubulekas I, Förberg C, Le H, Hektor A, Neubauer P, Pragai Z, Harwood C, Ward A, Picon A, De Mattos JT, Postma P, Farewell A, Nyström T, Reeh S, Pedersen S, Larsson G. Sandén AM, et al. Among authors: neubauer p. Biotechnol Bioeng. 2003 Jan 20;81(2):158-66. doi: 10.1002/bit.10457. Biotechnol Bioeng. 2003. PMID: 12451552
Fermentation process for tetrameric human collagen prolyl 4-hydroxylase in Escherichia coli: improvement by gene optimisation of the PDI/beta subunit and repeated addition of the inducer anhydrotetracycline.
Neubauer A, Soini J, Bollok M, Zenker M, Sandqvist J, Myllyharju J, Neubauer P. Neubauer A, et al. Among authors: neubauer p. J Biotechnol. 2007 Feb 1;128(2):308-21. doi: 10.1016/j.jbiotec.2006.10.017. Epub 2006 Nov 7. J Biotechnol. 2007. PMID: 17126943
Cytoplasmic production of an active human C-P4H has recently been described in the Origami (trxB gor) mutant Escherichia coli using a bicistronic vector with independent control of the alpha and PDI/beta subunit expression by the tetA and T5-lac promoters, respectively, enabling …
Cytoplasmic production of an active human C-P4H has recently been described in the Origami (trxB gor) mutant Escherichia coli using a bicist …
327 results