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Controlling genetic heterogeneity in gene-edited hematopoietic stem cells by single-cell expansion.
Cell Stem Cell. 2023 Jul 6;30(7):987-1000.e8. doi: 10.1016/j.stem.2023.06.002. Epub 2023 Jun 28.
Cell Stem Cell. 2023.
PMID: 37385251
Free PMC article.
Chemically defined cytokine-free expansion of human haematopoietic stem cells.
Sakurai M, Ishitsuka K, Ito R, Wilkinson AC, Kimura T, Mizutani E, Nishikii H, Sudo K, Becker HJ, Takemoto H, Sano T, Kataoka K, Takahashi S, Nakamura Y, Kent DG, Iwama A, Chiba S, Okamoto S, Nakauchi H, Yamazaki S.
Sakurai M, et al.
Nature. 2023 Mar;615(7950):127-133. doi: 10.1038/s41586-023-05739-9. Epub 2023 Feb 22.
Nature. 2023.
PMID: 36813966
Free article.
Here we report the establishment of a culture system that allows the long-term ex vivo expansion of human HSCs, achieved through the complete replacement of exogenous cytokines and albumin with chemical agonists and a caprolactam-based polymer. ...
Here we report the establishment of a culture system that allows the long-term ex vivo expansion of human HSCs, achieved through the complet …
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