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An efficient system for stable markerless integration of large biosynthetic gene clusters into Streptomyces chromosomes.
Csolleiova D, Knirschova R, Rezuchova B, Homerova D, Sevcikova B, Matulova M, Núñez LE, Novakova R, Feckova L, Javorova R, Cortés J, Kormanec J. Csolleiova D, et al. Among authors: nunez le. Appl Microbiol Biotechnol. 2021 Mar;105(5):2123-2137. doi: 10.1007/s00253-021-11161-w. Epub 2021 Feb 10. Appl Microbiol Biotechnol. 2021. PMID: 33564923
An efficient blue-white screening system for markerless deletions and stable integrations in Streptomyces chromosomes based on the blue pigment indigoidine biosynthetic gene bpsA.
Rezuchova B, Homerova D, Sevcikova B, Núñez LE, Novakova R, Feckova L, Skultety L, Cortés J, Kormanec J. Rezuchova B, et al. Among authors: nunez le. Appl Microbiol Biotechnol. 2018 Dec;102(23):10231-10244. doi: 10.1007/s00253-018-9393-7. Epub 2018 Sep 26. Appl Microbiol Biotechnol. 2018. PMID: 30259098
Genetic Engineering in Combination with Semi-Synthesis Leads to a New Route for Gram-Scale Production of the Immunosuppressive Natural Product Brasilicardin A.
Botas A, Eitel M, Schwarz PN, Buchmann A, Costales P, Núñez LE, Cortés J, Morís F, Krawiec M, Wolański M, Gust B, Rodriguez M, Fischer WN, Jandeleit B, Zakrzewska-Czerwińska J, Wohlleben W, Stegmann E, Koch P, Méndez C, Gross H. Botas A, et al. Among authors: nunez le. Angew Chem Int Ed Engl. 2021 Jun 7;60(24):13536-13541. doi: 10.1002/anie.202015852. Epub 2021 May 1. Angew Chem Int Ed Engl. 2021. PMID: 33768597 Free PMC article.
High-Quality Draft Genome Sequence of the Actinobacterium Nocardia terpenica IFM 0406, Producer of the Immunosuppressant Brasilicardins, Using Illumina and PacBio Technologies.
Buchmann A, Eitel M, Koch P, Schwarz PN, Stegmann E, Wohlleben W, Wolański M, Krawiec M, Zakrzewska-Czerwińska J, Méndez C, Botas A, Núñez LE, Morís F, Cortés J, Gross H. Buchmann A, et al. Among authors: nunez le. Genome Announc. 2016 Dec 15;4(6):e01391-16. doi: 10.1128/genomeA.01391-16. Genome Announc. 2016. PMID: 27979943 Free PMC article.
A novel LysR-type regulator negatively affects biosynthesis of the immunosuppressant brasilicardin.
Wolański M, Krawiec M, Schwarz PN, Stegmann E, Wohlleben W, Buchmann A, Gross H, Eitel M, Koch P, Botas A, Méndez C, Núñez LE, Morís F, Cortés J, Zakrzewska-Czerwińska J. Wolański M, et al. Among authors: nunez le. Eng Life Sci. 2020 Nov 4;21(1-2):4-18. doi: 10.1002/elsc.202000038. eCollection 2021 Jan. Eng Life Sci. 2020. PMID: 33531886 Free PMC article.
46 results