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Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins.
Malm M, Kuo CC, Barzadd MM, Mebrahtu A, Wistbacka N, Razavi R, Volk AL, Lundqvist M, Kotol D, Tegel H, Hober S, Edfors F, Gräslund T, Chotteau V, Field R, Varley PG, Roth RG, Lewis NE, Hatton D, Rockberg J. Malm M, et al. Metab Eng. 2022 Jul;72:171-187. doi: 10.1016/j.ymben.2022.03.009. Epub 2022 Mar 14. Metab Eng. 2022. PMID: 35301123 Free PMC article.
The human secretome.
Uhlén M, Karlsson MJ, Hober A, Svensson AS, Scheffel J, Kotol D, Zhong W, Tebani A, Strandberg L, Edfors F, Sjöstedt E, Mulder J, Mardinoglu A, Berling A, Ekblad S, Dannemeyer M, Kanje S, Rockberg J, Lundqvist M, Malm M, Volk AL, Nilsson P, Månberg A, Dodig-Crnkovic T, Pin E, Zwahlen M, Oksvold P, von Feilitzen K, Häussler RS, Hong MG, Lindskog C, Ponten F, Katona B, Vuu J, Lindström E, Nielsen J, Robinson J, Ayoglu B, Mahdessian D, Sullivan D, Thul P, Danielsson F, Stadler C, Lundberg E, Bergström G, Gummesson A, Voldborg BG, Tegel H, Hober S, Forsström B, Schwenk JM, Fagerberg L, Sivertsson Å. Uhlén M, et al. Among authors: malm m. Sci Signal. 2019 Nov 26;12(609):eaaz0274. doi: 10.1126/scisignal.aaz0274. Sci Signal. 2019. PMID: 31772123
Autophagy and intracellular product degradation genes identified by systems biology analysis reduce aggregation of bispecific antibody in CHO cells.
Barzadd MM, Lundqvist M, Harris C, Malm M, Volk AL, Thalén N, Chotteau V, Grassi L, Smith A, Abadi ML, Lambiase G, Gibson S, Hatton D, Rockberg J. Barzadd MM, et al. Among authors: malm m. N Biotechnol. 2022 May 25;68:68-76. doi: 10.1016/j.nbt.2022.01.010. Epub 2022 Feb 2. N Biotechnol. 2022. PMID: 35123066 Free article.
Enhanced metabolism and negative regulation of ER stress support higher erythropoietin production in HEK293 cells.
Saghaleyni R, Malm M, Moruzzi N, Zrimec J, Razavi R, Wistbacka N, Thorell H, Pintar A, Hober A, Edfors F, Chotteau V, Berggren PO, Grassi L, Zelezniak A, Svensson T, Hatton D, Nielsen J, Robinson JL, Rockberg J. Saghaleyni R, et al. Among authors: malm m. Cell Rep. 2022 Jun 14;39(11):110936. doi: 10.1016/j.celrep.2022.110936. Cell Rep. 2022. PMID: 35705050 Free article.
Tuning of CHO secretional machinery improve activity of secreted therapeutic sulfatase 150-fold.
Thalén NB, Barzadd MM, Lundqvist M, Rodhe J, Andersson M, Bidkhori G, Possner D, Su C, Nilsson J, Eisenhut P, Malm M, Karlsson A, Vestin J, Forsberg J, Nordling E, Mardinoglu A, Volk AL, Sandegren A, Rockberg J. Thalén NB, et al. Among authors: malm m. Metab Eng. 2024 Jan;81:157-166. doi: 10.1016/j.ymben.2023.12.003. Epub 2023 Dec 9. Metab Eng. 2024. PMID: 38081506 Free article.
141 results