Role of tyrosine 129 in the active site of spinach glycolate oxidase.
Macheroux P, Kieweg V, Massey V, Söderlind E, Stenberg K, Lindqvist Y.
Macheroux P, et al.
Eur J Biochem. 1993 May 1;213(3):1047-54. doi: 10.1111/j.1432-1033.1993.tb17852.x.
Eur J Biochem. 1993.
PMID: 8504801
Free article.
The spectrum indicates that the flavin is in its normal protonated form, i.e. the Y129F mutant does not lower the pKa of the N(3) of oxidized flavin as does the wild-type enzyme [Macheroux, P., Massey, V., Thiele, D. J., and Volokita, M. (1991) Biochemistry 30, 4612 …
The spectrum indicates that the flavin is in its normal protonated form, i.e. the Y129F mutant does not lower the pKa of the N(3) of oxidize …