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In conversation with Peter Macheroux.
Skourti E, Macheroux P. Skourti E, et al. Among authors: macheroux p. FEBS J. 2023 May;290(9):2208-2213. doi: 10.1111/febs.16646. Epub 2022 Oct 29. FEBS J. 2023. PMID: 36308323 Free article.
Role of tyrosine 129 in the active site of spinach glycolate oxidase.
Macheroux P, Kieweg V, Massey V, Söderlind E, Stenberg K, Lindqvist Y. Macheroux P, et al. Eur J Biochem. 1993 May 1;213(3):1047-54. doi: 10.1111/j.1432-1033.1993.tb17852.x. Eur J Biochem. 1993. PMID: 8504801 Free article.
The spectrum indicates that the flavin is in its normal protonated form, i.e. the Y129F mutant does not lower the pKa of the N(3) of oxidized flavin as does the wild-type enzyme [Macheroux, P., Massey, V., Thiele, D. J., and Volokita, M. (1991) Biochemistry 30, 4612 …
The spectrum indicates that the flavin is in its normal protonated form, i.e. the Y129F mutant does not lower the pKa of the N(3) of oxidize …
166 results