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Molecular engineering of a cryptic epitope in Spike RBD improves manufacturability and neutralizing breadth against SARS-CoV-2 variants.
Vaccine. 2023 Jan 27;41(5):1108-1118. doi: 10.1016/j.vaccine.2022.12.062. Epub 2022 Dec 29.
Vaccine. 2023.
PMID: 36610932
Free PMC article.
Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor-binding domain in engineered Komagataella phaffii.
Dalvie NC, Biedermann AM, Rodriguez-Aponte SA, Naranjo CA, Rao HD, Rajurkar MP, Lothe RR, Shaligram US, Johnston RS, Crowell LE, Castelino S, Tracey MK, Whittaker CA, Love JC.
Dalvie NC, et al. Among authors: lothe rr.
Biotechnol Bioeng. 2022 Feb;119(2):657-662. doi: 10.1002/bit.27979. Epub 2021 Nov 15.
Biotechnol Bioeng. 2022.
PMID: 34780057
Free PMC article.
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SARS-CoV-2 receptor binding domain displayed on HBsAg virus-like particles elicits protective immunity in macaques.
Dalvie NC, Tostanoski LH, Rodriguez-Aponte SA, Kaur K, Bajoria S, Kumru OS, Martinot AJ, Chandrashekar A, McMahan K, Mercado NB, Yu J, Chang A, Giffin VM, Nampanya F, Patel S, Bowman L, Naranjo CA, Yun D, Flinchbaugh Z, Pessaint L, Brown R, Velasco J, Teow E, Cook A, Andersen H, Lewis MG, Camp DL, Silverman JM, Nagar GS, Rao HD, Lothe RR, Chandrasekharan R, Rajurkar MP, Shaligram US, Kleanthous H, Joshi SB, Volkin DB, Biswas S, Love JC, Barouch DH.
Dalvie NC, et al. Among authors: lothe rr.
Sci Adv. 2022 Mar 18;8(11):eabl6015. doi: 10.1126/sciadv.abl6015. Epub 2022 Mar 16.
Sci Adv. 2022.
PMID: 35294244
Free PMC article.
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RBD-VLP Vaccines Adjuvanted with Alum or SWE Protect K18-hACE2 Mice against SARS-CoV-2 VOC Challenge.
Wong TY, Russ BP, Lee KS, Miller OA, Kang J, Cooper M, Winters MT, Rodriguez-Aponte SA, Dalvie NC, Johnston RS, Rader NA, Wong ZY, Cyphert HA, Martinez I, Shaligram U, Batwal S, Lothe R, Chandrasekaran R, Nagar G, Rajurkar M, Rao H, Bevere JR, Barbier M, Love JC, Damron FH.
Wong TY, et al.
mSphere. 2022 Aug 31;7(4):e0024322. doi: 10.1128/msphere.00243-22. Epub 2022 Aug 15.
mSphere. 2022.
PMID: 35968964
Free PMC article.
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Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast.
Nagar G, Jain S, Rajurkar M, Lothe R, Rao H, Majumdar S, Gautam M, Rodriguez-Aponte SA, Crowell LE, Love JC, Dandekar P, Puranik A, Gairola S, Shaligram U, Jain R.
Nagar G, et al.
Vaccines (Basel). 2023 Oct 16;11(10):1602. doi: 10.3390/vaccines11101602.
Vaccines (Basel). 2023.
PMID: 37897004
Free PMC article.
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Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii.
Dalvie NC, Biedermann AM, Rodriguez-Aponte SA, Naranjo CA, Rao HD, Rajurkar MP, Lothe RR, Shaligram US, Johnston RS, Crowell LE, Castelino S, Tracey MK, Whittaker CA, Love JC.
Dalvie NC, et al. Among authors: lothe rr.
bioRxiv [Preprint]. 2021 Apr 15:2021.04.15.440035. doi: 10.1101/2021.04.15.440035.
bioRxiv. 2021.
PMID: 33880471
Free PMC article.
Updated.
Preprint.
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Purification of alpha-glucosidae and invertase from bakers' yeast on modified polymeric supports.
Lothe RR, Purohit SS, Shaikh SS, Malshe VC, Pandit AB.
Lothe RR, et al.
Bioseparation. 1999;8(6):293-306. doi: 10.1023/a:1008126628635.
Bioseparation. 1999.
PMID: 10786278
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Comments on the mechanism of microbial cell disruption in high-pressure and high-speed devices.
Shirgaonkar IZ, Lothe RR, Pandit AB.
Shirgaonkar IZ, et al. Among authors: lothe rr.
Biotechnol Prog. 1998 Jul;14(4):657-60. doi: 10.1021/bp980052g.
Biotechnol Prog. 1998.
PMID: 9694691
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