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Receptor-binding domain recombinant protein on alum-CpG induces broad protection against SARS-CoV-2 variants of concern.
Pollet J, Strych U, Chen WH, Versteeg L, Keegan B, Zhan B, Wei J, Liu Z, Lee J, Kundu R, Adhikari R, Poveda C, Jose Villar M, Rani Thimmiraju S, Lopez B, Gillespie PM, Ronca S, Kimata JT, Reers M, Paradkar V, Hotez PJ, Elena Bottazzi M. Pollet J, et al. Among authors: lee j. Vaccine. 2022 Jun 9;40(26):3655-3663. doi: 10.1016/j.vaccine.2022.05.007. Epub 2022 May 8. Vaccine. 2022. PMID: 35568591 Free PMC article.
Heterogeneous antibodies against SARS-CoV-2 spike receptor binding domain and nucleocapsid with implications for COVID-19 immunity.
McAndrews KM, Dowlatshahi DP, Dai J, Becker LM, Hensel J, Snowden LM, Leveille JM, Brunner MR, Holden KW, Hopkins NS, Harris AM, Kumpati J, Whitt MA, Lee JJ, Ostrosky-Zeichner LL, Papanna R, LeBleu VS, Allison JP, Kalluri R. McAndrews KM, et al. Among authors: lee jj. JCI Insight. 2020 Sep 17;5(18):e142386. doi: 10.1172/jci.insight.142386. JCI Insight. 2020. PMID: 32796155 Free PMC article.
SARS-CoV-2 RBD219-N1C1: A Yeast-Expressed SARS-CoV-2 Recombinant Receptor-Binding Domain Candidate Vaccine Stimulates Virus Neutralizing Antibodies and T-cell Immunity in Mice.
Pollet J, Chen WH, Versteeg L, Keegan B, Zhan B, Wei J, Liu Z, Lee J, Kundu R, Adhikari R, Poveda C, Villar MJ, de Araujo Leao AC, Rivera JA, Momin Z, Gillespie PM, Kimata JT, Strych U, Hotez PJ, Bottazzi ME. Pollet J, et al. Among authors: lee j. bioRxiv [Preprint]. 2021 Feb 9:2020.11.04.367359. doi: 10.1101/2020.11.04.367359. bioRxiv. 2021. PMID: 33173864 Free PMC article. Updated. Preprint.
Genetic modification to design a stable yeast-expressed recombinant SARS-CoV-2 receptor binding domain as a COVID-19 vaccine candidate.
Chen WH, Wei J, Kundu RT, Adhikari R, Liu Z, Lee J, Versteeg L, Poveda C, Keegan B, Villar MJ, de Araujo Leao AC, Rivera JA, Gillespie PM, Pollet J, Strych U, Zhan B, Hotez PJ, Bottazzi ME. Chen WH, et al. Among authors: lee j. Biochim Biophys Acta Gen Subj. 2021 Jun;1865(6):129893. doi: 10.1016/j.bbagen.2021.129893. Epub 2021 Mar 14. Biochim Biophys Acta Gen Subj. 2021. PMID: 33731300 Free PMC article.
SARS‑CoV-2 RBD219-N1C1: A yeast-expressed SARS-CoV-2 recombinant receptor-binding domain candidate vaccine stimulates virus neutralizing antibodies and T-cell immunity in mice.
Pollet J, Chen WH, Versteeg L, Keegan B, Zhan B, Wei J, Liu Z, Lee J, Kundu R, Adhikari R, Poveda C, Villar MJ, de Araujo Leao AC, Altieri Rivera J, Momin Z, Gillespie PM, Kimata JT, Strych U, Hotez PJ, Bottazzi ME. Pollet J, et al. Among authors: lee j. Hum Vaccin Immunother. 2021 Aug 3;17(8):2356-2366. doi: 10.1080/21645515.2021.1901545. Epub 2021 Apr 13. Hum Vaccin Immunother. 2021. PMID: 33847226 Free PMC article.
A yeast expressed RBD-based SARS-CoV-2 vaccine formulated with 3M-052-alum adjuvant promotes protective efficacy in non-human primates.
Pino M, Abid T, Pereira Ribeiro S, Edara VV, Floyd K, Smith JC, Latif MB, Pacheco-Sanchez G, Dutta D, Wang S, Gumber S, Kirejczyk S, Cohen J, Stammen RL, Jean SM, Wood JS, Connor-Stroud F, Pollet J, Chen WH, Wei J, Zhan B, Lee J, Liu Z, Strych U, Shenvi N, Easley K, Weiskopf D, Sette A, Pollara J, Mielke D, Gao H, Eisel N, LaBranche CC, Shen X, Ferrari G, Tomaras GD, Montefiori DC, Sekaly RP, Vanderford TH, Tomai MA, Fox CB, Suthar MS, Kozlowski PA, Hotez PJ, Paiardini M, Bottazzi ME, Kasturi SP. Pino M, et al. Among authors: lee j. Sci Immunol. 2021 Jul 15;6(61):eabh3634. doi: 10.1126/sciimmunol.abh3634. Sci Immunol. 2021. PMID: 34266981 Free PMC article.
Yeast-expressed recombinant SARS-CoV-2 receptor binding domain RBD203-N1 as a COVID-19 protein vaccine candidate.
Chen WH, Pollet J, Strych U, Lee J, Liu Z, Kundu RT, Versteeg L, Villar MJ, Adhikari R, Wei J, Poveda C, Keegan B, Bailey AO, Chen YL, Gillespie PM, Kimata JT, Zhan B, Hotez PJ, Bottazzi ME. Chen WH, et al. Among authors: lee j. Protein Expr Purif. 2022 Feb;190:106003. doi: 10.1016/j.pep.2021.106003. Epub 2021 Oct 21. Protein Expr Purif. 2022. PMID: 34688919 Free PMC article.
From concept to delivery: a yeast-expressed recombinant protein-based COVID-19 vaccine technology suitable for global access.
Hotez PJ, Adhikari R, Chen WH, Chen YL, Gillespie P, Islam NY, Keegan B, Tyagi Kundu R, Lee J, Liu Z, Kimata JT, Oezguen N, Pollet J, Poveda C, Razavi K, Ronca SE, Strych U, Thimmiraju SR, Versteeg L, Villar-Mondragon MJ, Wei J, Zhan B, Bottazzi ME. Hotez PJ, et al. Among authors: lee j. Expert Rev Vaccines. 2023 Jan-Dec;22(1):495-500. doi: 10.1080/14760584.2023.2217917. Expert Rev Vaccines. 2023. PMID: 37252854 Free article.
A Recombinant Protein XBB.1.5 RBD/Alum/CpG Vaccine Elicits High Neutralizing Antibody Titers against Omicron Subvariants of SARS-CoV-2.
Thimmiraju SR, Adhikari R, Villar MJ, Lee J, Liu Z, Kundu R, Chen YL, Sharma S, Ghei K, Keegan B, Versteeg L, Gillespie PM, Ciciriello A, Islam NY, Poveda C, Uzcategui N, Chen WH, Kimata JT, Zhan B, Strych U, Bottazzi ME, Hotez PJ, Pollet J. Thimmiraju SR, et al. Among authors: lee j. Vaccines (Basel). 2023 Oct 1;11(10):1557. doi: 10.3390/vaccines11101557. Vaccines (Basel). 2023. PMID: 37896960 Free PMC article.
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