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Anti-CRISPRs: Protein Inhibitors of CRISPR-Cas Systems.
Davidson AR, Lu WT, Stanley SY, Wang J, Mejdani M, Trost CN, Hicks BT, Lee J, Sontheimer EJ. Davidson AR, et al. Among authors: lee j. Annu Rev Biochem. 2020 Jun 20;89:309-332. doi: 10.1146/annurev-biochem-011420-111224. Epub 2020 Mar 18. Annu Rev Biochem. 2020. PMID: 32186918 Free PMC article. Review.
Naturally Occurring Off-Switches for CRISPR-Cas9.
Pawluk A, Amrani N, Zhang Y, Garcia B, Hidalgo-Reyes Y, Lee J, Edraki A, Shah M, Sontheimer EJ, Maxwell KL, Davidson AR. Pawluk A, et al. Among authors: lee j. Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8. Cell. 2016. PMID: 27984730 Free PMC article.
Type II-C CRISPR-Cas9 Biology, Mechanism, and Application.
Mir A, Edraki A, Lee J, Sontheimer EJ. Mir A, et al. Among authors: lee j. ACS Chem Biol. 2018 Feb 16;13(2):357-365. doi: 10.1021/acschembio.7b00855. Epub 2017 Dec 20. ACS Chem Biol. 2018. PMID: 29202216 Free PMC article. Review.
Potent Cas9 Inhibition in Bacterial and Human Cells by AcrIIC4 and AcrIIC5 Anti-CRISPR Proteins.
Lee J, Mir A, Edraki A, Garcia B, Amrani N, Lou HE, Gainetdinov I, Pawluk A, Ibraheim R, Gao XD, Liu P, Davidson AR, Maxwell KL, Sontheimer EJ. Lee J, et al. mBio. 2018 Dec 4;9(6):e02321-18. doi: 10.1128/mBio.02321-18. mBio. 2018. PMID: 30514786 Free PMC article.
J. Sontheimer and A. R. Davidson, Curr Opin Microbiol 37:120-127, 2017, https://doi.org/10.1016/j.mib.2017.06.003). ...In this work, we report two novel anti-CRISPR families in strains of Haemophilus parainfluenzae and Simonsiella muelleri, both of which harbor type …
J. Sontheimer and A. R. Davidson, Curr Opin Microbiol 37:120-127, 2017, https://doi.org/10.1016/j.mib.2017.06.003). ...In this …
Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing.
Garcia B, Lee J, Edraki A, Hidalgo-Reyes Y, Erwood S, Mir A, Trost CN, Seroussi U, Stanley SY, Cohn RD, Claycomb JM, Sontheimer EJ, Maxwell KL, Davidson AR. Garcia B, et al. Among authors: lee j. Cell Rep. 2019 Nov 12;29(7):1739-1746.e5. doi: 10.1016/j.celrep.2019.10.017. Cell Rep. 2019. PMID: 31722192 Free PMC article.
An engineered ScCas9 with broad PAM range and high specificity and activity.
Chatterjee P, Jakimo N, Lee J, Amrani N, Rodríguez T, Koseki SRT, Tysinger E, Qing R, Hao S, Sontheimer EJ, Jacobson J. Chatterjee P, et al. Among authors: lee j. Nat Biotechnol. 2020 Oct;38(10):1154-1158. doi: 10.1038/s41587-020-0517-0. Epub 2020 May 11. Nat Biotechnol. 2020. PMID: 32393822
A Cas9 with PAM recognition for adenine dinucleotides.
Chatterjee P, Lee J, Nip L, Koseki SRT, Tysinger E, Sontheimer EJ, Jacobson JM, Jakimo N. Chatterjee P, et al. Among authors: lee j. Nat Commun. 2020 May 18;11(1):2474. doi: 10.1038/s41467-020-16117-8. Nat Commun. 2020. PMID: 32424114 Free PMC article.
Efficient Homology-Directed Repair with Circular Single-Stranded DNA Donors.
Iyer S, Mir A, Vega-Badillo J, Roscoe BP, Ibraheim R, Zhu LJ, Lee J, Liu P, Luk K, Mintzer E, Guo D, Soares de Brito J, Emerson CP Jr, Zamore PD, Sontheimer EJ, Wolfe SA. Iyer S, et al. Among authors: lee j. CRISPR J. 2022 Oct;5(5):685-701. doi: 10.1089/crispr.2022.0058. Epub 2022 Sep 7. CRISPR J. 2022. PMID: 36070530 Free PMC article.
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