Lakey JH - Search Results

1

Probing the structural basis for the difference in thermostability displayed by family 10 xylanases.

Xie H, Flint J, Vardakou M, Lakey JH, Lewis RJ, Gilbert HJ, Dumon C. Xie H, et al. Among authors: lakey jh. J Mol Biol. 2006 Jun 30;360(1):157-67. doi: 10.1016/j.jmb.2006.05.002. Epub 2006 May 15. J Mol Biol. 2006. PMID: 16762367

2

The use of forced protein evolution to investigate and improve stability of family 10 xylanases. The production of Ca2+-independent stable xylanases.

Andrews SR, Taylor EJ, Pell G, Vincent F, Ducros VM, Davies GJ, Lakey JH, Gilbert HJ. Andrews SR, et al. Among authors: lakey jh. J Biol Chem. 2004 Dec 24;279(52):54369-79. doi: 10.1074/jbc.M409044200. Epub 2004 Sep 27. J Biol Chem. 2004. PMID: 15452124 Free article.

3

Engineering hyperthermostability into a GH11 xylanase is mediated by subtle changes to protein structure.

Dumon C, Varvak A, Wall MA, Flint JE, Lewis RJ, Lakey JH, Morland C, Luginbühl P, Healey S, Todaro T, DeSantis G, Sun M, Parra-Gessert L, Tan X, Weiner DP, Gilbert HJ. Dumon C, et al. Among authors: lakey jh. J Biol Chem. 2008 Aug 15;283(33):22557-64. doi: 10.1074/jbc.M800936200. Epub 2008 May 30. J Biol Chem. 2008. PMID: 18515360 Free article.

4

Role of hydrogen bonding in the interaction between a xylan binding module and xylan.

Xie H, Bolam DN, Nagy T, Szabó L, Cooper A, Simpson PJ, Lakey JH, Williamson MP, Gilbert HJ. Xie H, et al. Among authors: lakey jh. Biochemistry. 2001 May 15;40(19):5700-7. doi: 10.1021/bi010034z. Biochemistry. 2001. PMID: 11341835

5

The alpha-glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants.

Nagy T, Nurizzo D, Davies GJ, Biely P, Lakey JH, Bolam DN, Gilbert HJ. Nagy T, et al. Among authors: lakey jh. J Biol Chem. 2003 May 30;278(22):20286-92. doi: 10.1074/jbc.M302205200. Epub 2003 Mar 24. J Biol Chem. 2003. PMID: 12654910 Free article.

6

Key residues in subsite F play a critical role in the activity of Pseudomonas fluorescens subspecies cellulosa xylanase A against xylooligosaccharides but not against highly polymeric substrates such as xylan.

Charnock SJ, Lakey JH, Virden R, Hughes N, Sinnott ML, Hazlewood GP, Pickersgill R, Gilbert HJ. Charnock SJ, et al. Among authors: lakey jh. J Biol Chem. 1997 Jan 31;272(5):2942-51. doi: 10.1074/jbc.272.5.2942. J Biol Chem. 1997. PMID: 9006940 Free article.

7

Evidence that galactanase A from Pseudomonas fluorescens subspecies cellulosa is a retaining family 53 glycosyl hydrolase in which E161 and E270 are the catalytic residues.

Braithwaite KL, Barna T, Spurway TD, Charnock SJ, Black GW, Hughes N, Lakey JH, Virden R, Hazlewood GP, Henrissat B, Gilbert HJ. Braithwaite KL, et al. Among authors: lakey jh. Biochemistry. 1997 Dec 9;36(49):15489-500. doi: 10.1021/bi9712394. Biochemistry. 1997. PMID: 9398278

8

Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues.

Bolam DN, Hughes N, Virden R, Lakey JH, Hazlewood GP, Henrissat B, Braithwaite KL, Gilbert HJ. Bolam DN, et al. Among authors: lakey jh. Biochemistry. 1996 Dec 17;35(50):16195-204. doi: 10.1021/bi961866d. Biochemistry. 1996. PMID: 8973192

9

Substrate specificity in glycoside hydrolase family 10. Tyrosine 87 and leucine 314 play a pivotal role in discriminating between glucose and xylose binding in the proximal active site of Pseudomonas cellulosa xylanase 10A.

Andrews SR, Charnock SJ, Lakey JH, Davies GJ, Claeyssens M, Nerinckx W, Underwood M, Sinnott ML, Warren RA, Gilbert HJ. Andrews SR, et al. Among authors: lakey jh. J Biol Chem. 2000 Jul 28;275(30):23027-33. doi: 10.1074/jbc.M000128200. J Biol Chem. 2000. PMID: 10767281 Free article.

10

Alhydrogel® adjuvant, ultrasonic dispersion and protein binding: a TEM and analytical study.

Harris JR, Soliakov A, Lewis RJ, Depoix F, Watkinson A, Lakey JH. Harris JR, et al. Among authors: lakey jh. Micron. 2012 Feb;43(2-3):192-200. doi: 10.1016/j.micron.2011.07.012. Epub 2011 Jul 27. Micron. 2012. PMID: 21831642

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