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Harnessing secretory pathway differences between HEK293 and CHO to rescue production of difficult to express proteins.
Malm M, Kuo CC, Barzadd MM, Mebrahtu A, Wistbacka N, Razavi R, Volk AL, Lundqvist M, Kotol D, Tegel H, Hober S, Edfors F, Gräslund T, Chotteau V, Field R, Varley PG, Roth RG, Lewis NE, Hatton D, Rockberg J. Malm M, et al. Among authors: kuo cc. Metab Eng. 2022 Jul;72:171-187. doi: 10.1016/j.ymben.2022.03.009. Epub 2022 Mar 14. Metab Eng. 2022. PMID: 35301123 Free PMC article.
Combinatorial CRISPR-Cas9 screens for de novo mapping of genetic interactions.
Shen JP, Zhao D, Sasik R, Luebeck J, Birmingham A, Bojorquez-Gomez A, Licon K, Klepper K, Pekin D, Beckett AN, Sanchez KS, Thomas A, Kuo CC, Du D, Roguev A, Lewis NE, Chang AN, Kreisberg JF, Krogan N, Qi L, Ideker T, Mali P. Shen JP, et al. Among authors: kuo cc. Nat Methods. 2017 Jun;14(6):573-576. doi: 10.1038/nmeth.4225. Epub 2017 Mar 20. Nat Methods. 2017. PMID: 28319113 Free PMC article.
An enhanced CRISPR repressor for targeted mammalian gene regulation.
Yeo NC, Chavez A, Lance-Byrne A, Chan Y, Menn D, Milanova D, Kuo CC, Guo X, Sharma S, Tung A, Cecchi RJ, Tuttle M, Pradhan S, Lim ET, Davidsohn N, Ebrahimkhani MR, Collins JJ, Lewis NE, Kiani S, Church GM. Yeo NC, et al. Among authors: kuo cc. Nat Methods. 2018 Aug;15(8):611-616. doi: 10.1038/s41592-018-0048-5. Epub 2018 Jul 16. Nat Methods. 2018. PMID: 30013045 Free PMC article.
Combating viral contaminants in CHO cells by engineering innate immunity.
Chiang AWT, Li S, Kellman BP, Chattopadhyay G, Zhang Y, Kuo CC, Gutierrez JM, Ghazi F, Schmeisser H, Ménard P, Bjørn SP, Voldborg BG, Rosenberg AS, Puig M, Lewis NE. Chiang AWT, et al. Among authors: kuo cc. Sci Rep. 2019 Jun 20;9(1):8827. doi: 10.1038/s41598-019-45126-x. Sci Rep. 2019. PMID: 31222165 Free PMC article.
In situ detection of protein interactions for recombinant therapeutic enzymes.
Samoudi M, Kuo CC, Robinson CM, Shams-Ud-Doha K, Schinn SM, Kol S, Weiss L, Petersen Bjorn S, Voldborg BG, Rosa Campos A, Lewis NE. Samoudi M, et al. Among authors: kuo cc. Biotechnol Bioeng. 2021 Feb;118(2):890-904. doi: 10.1002/bit.27621. Epub 2020 Nov 24. Biotechnol Bioeng. 2021. PMID: 33169829 Free PMC article.
1,518 results